The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Heparin is required for cell-free binding of basic fibroblast growth factor to a soluble receptor and for mitogenesis in whole cells.
Open accessAbstract
Heparin is required for the binding of basic fibroblast growth factor (bFGF) to high-affinity receptors on cells deficient in cell surface heparan sulfate proteoglycan. So that this heparin requirement could be evaluated in the absence of other cell surface molecules, we designed a simple assay based on a genetically engineered soluble form of murine FGF receptor 1 (mFR1) tagged with placental alkaline phosphatase. Using this assay, we showed that FGF-receptor binding has an absolute requirement for heparin. By using a cytokine-dependent lymphoid cell line engineered to express mFR1, we also showed that FGF-induced mitogenic activity is heparin dependent. Furthermore, we tested a series of small heparin oligosaccharides of defined lengths for their abilities to support bFGF-receptor binding and biologic activity. We found that a heparin oligosaccharide with as few as eight sugar residues is sufficient to support these activities. We also demonstrated that heparin facilitates FGF dimerization, a property that may be important for receptor activation.
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Cites
Signal transduction by receptors with tyrosine kinase activity
Ullrich, A., & Schlessinger, J. (1990) · Cell · doi:10.1016/0092-8674(90)90801-k · Cited by 5,435
Yayon, A., Klagsbrun, M., Esko, J. D., Leder, P., & Ornitz, D. M. (1991) · Cell · doi:10.1016/0092-8674(91)90512-w · Cited by 2,440
Self-citationRequirement of Heparan Sulfate for bFGF-Mediated Fibroblast Growth and Myoblast Differentiation
Rapraeger, A. C., Krufka, A., & Olwin, B. B. (1991) · Science · doi:10.1126/science.1646484 · Cited by 1,508
Proteoglycans as modulators of growth factor activities
Ruoslahti, E., & Yamaguchi, Y. (1991) · Cell · doi:10.1016/0092-8674(91)90308-l · Cited by 1,343
Structural Characterization and Biological Functions of Fibroblast Growth Factor
GOSPODAROWICZ, D., FERRARA, N., SCHWEIGERER, L., & NEUFELD, G. (1987) · Endocrine Reviews · doi:10.1210/edrv-8-2-95 · Cited by 1,273
A heparin-binding growth factor secreted by macrophage-like cells that is related to EGF
Higashiyama, S., Abraham, J. A., Miller, J., Fiddes, J. C., & Klagsbrun, M. (1991) · Science · doi:10.1126/science.1840698 · Cited by 1,127
Karasuyama, H., & Melchers, F. (1988) · European Journal of Immunology · doi:10.1002/eji.1830180115 · Cited by 1,065
Vlodavsky, I., Folkman, J., Sullivan, R., Fridman, R., Ishai-Michaeli, R., Sasse, J., & Klagsbrun, M. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.8.2292 · Cited by 1,032
Heparan sulphate bound growth factors: a mechanism for stromal cell mediated haemopoiesis
Roberts, R., Gallagher, J., Spooncer, E., Alien, T. D., Bloomfield, F., & Dexter, T. M. (1988) · Nature · doi:10.1038/332376a0 · Cited by 807
The kit ligand: A cell surface molecule altered in steel mutant fibroblasts
Flanagan, J. G., & Leder, P. (1990) · Cell · doi:10.1016/0092-8674(90)90299-t · Cited by 748
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The Fibroblast Growth Factor signaling pathway
Ornitz, D. M., & Itoh, N. (2015) · WIREs Developmental Biology · doi:10.1002/wdev.176 · Cited by 2,050
Self-citationThe FGF family: biology, pathophysiology and therapy
Beenken, A., & Mohammadi, M. (2009) · Nature Reviews Drug Discovery · doi:10.1038/nrd2792 · Cited by 1,945
Receptor Specificity of the Fibroblast Growth Factor Family
Ornitz, D. M., Xu, J., Colvin, J. S., McEwen, D. G., MacArthur, C. A., Coulier, F., Gao, G., & Goldfarb, M. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.25.15292 · Cited by 1,871
Self-citationFibroblast growth factors, their receptors and signaling
Powers, C. J., McLeskey, S. W., & Wellstein, A. (2000) · Endocrine-related cancer · doi:10.1677/erc.0.0070165 · Cited by 1,332
Schlessinger, J., Plotnikov, A. N., Ibrahimi, O. A., Eliseenkova, A. V., Yeh, B. K., Yayon, A., Linhardt, R. J., & Mohammadi, M. (2000) · Molecular Cell · doi:10.1016/s1097-2765(00)00073-3 · Cited by 1,199
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Metadata
- Authors
- Ornitz, D. M., Yayon, A., Flanagan, J. G., Svahn, C. M., Levi, E., & Leder, P.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1992-01
- Volume
- 12
- Issue
- 1
- Pages
- 240-247
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2167544720
- Cited by
- 632
- References
- 36
- FWCI
- 16.03
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Heparin is required for cell-free binding of basic fibroblast growth factor to a soluble receptor and for mitogenesis in whole cells. https://app.cochat.ai/papers/10.1128/mcb.12.1.240
- The paper
- Ornitz, D. M., Yayon, A., Flanagan, J. G., Svahn, C. M., Levi, E., & Leder, P. (1992). Heparin is required for cell-free binding of basic fibroblast growth factor to a soluble receptor and for mitogenesis in whole cells. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.12.1.240
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
