The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Evidence that the N-terminal domain of nonstructural protein NS3 from yellow fever virus is a serine protease responsible for site-specific cleavages in the viral polyprotein.
Open accessAbstract
Sequence homology and molecular modeling studies have suggested that the N-terminal one-third of the flavirvirus nonstructural protein NS3 functions as a trypsin-like serine protease. To examine the putative proteolytic activity of NS3, segments of the yellow fever virus genome were subcloned into plasmid transcription/translation vectors and cell-free translation products were characterized. The results suggest that a protease activity encoded within NS2B and the N-terminal one-third of yellow fever virus NS3 is capable of cis-acting site-specific proteolysis at the NS2B-NS3 cleavage site and dilution-insensitive cleavage of the NS2A-NS2B site. Site-directed mutagenesis of the His-53, Asp-77, and Ser-138 residues of NS3 that compose the proposed catalytic triad implicates this domain as a serine protease. Infectious virus was not recovered from mammalian cells transfected with RNAs transcribed from full-length yellow fever virus cDNA templates containing mutations at Ser-138 (which abolish or dramatically reduce protease activity in vitro), suggesting that the protease is required for viral replication.
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Cites
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Self-citationStructure of human pancreatic lipase
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Cited by
Molecular biology of flaviviruses
Lindenbach, B. D., & Rice, C. M. (2003) · Advances in Virus Research · doi:10.1016/s0065-3527(03)59002-9 · Cited by 853
Self-citation[2] Families of serine peptidases
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Biochemistry and Molecular Biology of Flaviviruses
Barrows, N. J., Campos, R. K., Liao, K.-C., Prasanth, K. R., Soto-Acosta, R., Yeh, S.-C., Schott-Lerner, G., Pompon, J., Sessions, O. M., Bradrick, S. S., et al. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00719 · Cited by 341
Blitvich, B., & Firth, A. (2015) · Viruses · doi:10.3390/v7041927 · Cited by 323
Metadata
- Authors
- Chambers, T. J., Weir, R. C., Grakoui, A., McCourt, D. W., Bazan, J. F., Fletterick, R. J., & Rice, C. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1990-11
- Volume
- 87
- Issue
- 22
- Pages
- 8898-8902
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2074188269
- Cited by
- 351
- References
- 24
- FWCI
- 21.17
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Evidence that the N-terminal domain of nonstructural protein NS3 from yellow fever virus is a serine protease responsible for site-specific cleavages in the viral polyprotein. https://app.cochat.ai/papers/10.1073/pnas.87.22.8898
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- Chambers, T. J., Weir, R. C., Grakoui, A., McCourt, D. W., Bazan, J. F., Fletterick, R. J., & Rice, C. M. (1990). Evidence that the N-terminal domain of nonstructural protein NS3 from yellow fever virus is a serine protease responsible for site-specific cleavages in the viral polyprotein. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.87.22.8898
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