The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Apparent hydroxyl radical production by peroxynitrite: Implications for endothelial injury from nitric oxide and superoxide
Open accessAbstract
Superoxide dismutase reduces injury in many disease processes, implicating superoxide anion radical (O2-.) as a toxic species in vivo. A critical target of superoxide may be nitric oxide (NO.) produced by endothelium, macrophages, neutrophils, and brain synaptosomes. Superoxide and NO. are known to rapidly react to form the stable peroxynitrite anion (ONOO-). We have shown that peroxynitrite has a pKa of 7.49 +/- 0.06 at 37 degrees C and rapidly decomposes once protonated with a half-life of 1.9 sec at pH 7.4. Peroxynitrite decomposition generates a strong oxidant with reactivity similar to hydroxyl radical, as assessed by the oxidation of deoxyribose or dimethyl sulfoxide. Product yields indicative of hydroxyl radical were 5.1 +/- 0.1% and 24.3 +/- 1.0%, respectively, of added peroxynitrite. Product formation was not affected by the metal chelator diethyltriaminepentaacetic acid, suggesting that iron was not required to catalyze oxidation. In contrast, desferrioxamine was a potent, competitive inhibitor of peroxynitrite-initiated oxidation because of a direct reaction between desferrioxamine and peroxynitrite rather than by iron chelation. We propose that superoxide dismutase may protect vascular tissue stimulated to produce superoxide and NO. under pathological conditions by preventing the formation of peroxynitrite.
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Cites
Vascular endothelial cells synthesize nitric oxide from L-arginine
Palmer, R. M. J., Ashton, D., & Moncada, S. (1988) · Nature · doi:10.1038/333664a0 · Cited by 4,786
Superoxide anion is involved in the breakdown of endothelium-derived vascular relaxing factor
Gryglewski, R. J., Palmer, R. M. J., & Moncada, S. (1986) · Nature · doi:10.1038/320454a0 · Cited by 2,400
Endothelium‐derived relaxing and contracting factors
Furchgott, R. F., & Vanhoutte, P. M. (1989) · The FASEB Journal · doi:10.1096/fasebj.3.9.2545495 · Cited by 2,007
Macrophage oxidation of L-arginine to nitrite and nitrate: nitric oxide is an intermediate
Marletta, M. A., Yoon, P. S., Iyengar, R., Leaf, C. D., & Wishnok, J. S. (1988) · Biochemistry · doi:10.1021/bi00424a003 · Cited by 1,601
Knowles, R. G., Palacios‐Callender, M., Palmer, R. M. J., & Moncada, S. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.13.5159 · Cited by 1,431
Biological effects of the superoxide radical
Fridovich, I. (1986) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(86)90526-6 · Cited by 1,420
Direct measurement of free radical generation following reperfusion of ischemic myocardium.
Zweier, J. L., Flaherty, J. T., & Weisfeldt, M. L. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.5.1404 · Cited by 1,189
A spectrophotometric method for determination of catalase activity in small tissue samples
Johansson, L. H., & Borg, L. (1988) · Analytical Biochemistry · doi:10.1016/0003-2697(88)90554-4 · Cited by 1,097
Formation of a thiobarbituric‐acid‐reactive substance from deoxyribose in the presence of iron salts
Halliwell, B. B., & Gutteridge, J. M. C. (1981) · FEBS Letters · doi:10.1016/0014-5793(81)80114-7 · Cited by 832
McCall, T., Boughton-Smith, N. K., Palmer, R. M. J., Whittle, B. J. R., & Moncada, S. (1989) · Biochemical Journal · doi:10.1042/bj2610293 · Cited by 518
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Rosen, D. R., Siddique, T., Patterson, D., Figlewicz, D. A., Sapp, P., Hentati, A., Donaldson, D., Goto, J., O'Regan, J. P., Deng, H.-X., et al. (1993) · Nature · doi:10.1038/362059a0 · Cited by 7,062
The L-Arginine-Nitric Oxide Pathway
Epstein, F. H., Moncada, S., & Higgs, A. (1993) · New England Journal of Medicine · doi:10.1056/nejm199312303292706 · Cited by 6,678
Nitric Oxide and Peroxynitrite in Health and Disease
Pacher, P., Beckman, J. S., & Liaudet, L. (2007) · Physiological Reviews · doi:10.1152/physrev.00029.2006 · Cited by 6,390
Self-citationNitric oxide, superoxide, and peroxynitrite: the good, the bad, and ugly
Beckman, J. S., & Koppenol, W. H. (1996) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1996.271.5.c1424 · Cited by 5,513
Self-citationNitric oxide as a secretory product of mammalian cells
Nathan, C. (1992) · The FASEB Journal · doi:10.1096/fasebj.6.12.1381691 · Cited by 4,350
Metadata
- Authors
- Beckman, J. S., Beckman, T. W., Chen, J., Marshall, P. A., & Freeman, B. A.
- Affiliations
- University of Alabama at Birmingham (US)
- Department of Anesthesiology, University of Alabama, Birmingham 35233.
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1990-02
- Volume
- 87
- Issue
- 4
- Pages
- 1620-1624
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC53527
- OpenAlex
- W2058137293
- Cited by
- 7,029
- References
- 25
- Citations by year
- 2026: 33 · 2025: 43 · 2024: 76 · 2023: 75 · 2022: 84 · 2021: 83 · 2020: 96 · 2019: 94 · 2018: 104 · 2017: 123 · 2016: 123 · 2015: 124 · 2014: 179 · 2013: 160 · 2012: 187
- FWCI
- 67.23
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- American Heart Association
- National Institutes of Health (5r01ns024275-04)
- National Institute of Neurological Disorders and Stroke (NS 24338, NS24275)
- Topics
- Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Keywords
- peroxynitrite, hydroxyl radicals, superoxide dismutase, nitric oxide, superoxide, endothelial injury, peroxynitrite decomposition, deferoxamine, deoxyribose oxidation, dimethyl sulfoxide oxidation, pKa, oxidative stress
- MeSH terms
- Animals, Endothelium, Vascular, Free Radicals, Half-Life, Hydrogen-Ion Concentration, Hydroxides, Kinetics, Models, Biological, Nitrates, Nitric Oxide, Peroxides, Superoxides, Superoxide Dismutase, Hydroxyl Radical
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