The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction.
Open accessAbstract
The expression of two cytokines, granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 3 (IL-3), has been investigated in MLA-144 cells before and after induction with phorbol 12-myristate 13-acetate. We describe an adaptation of the polymerase chain reaction (PCR) for highly accurate quantitation of mRNA or DNA from a small number of cells. Aliquots of the PCR mixture containing cDNA copies of the RNA to be assayed were added to serial dilutions of a competitor DNA fragment that differed from the cDNA of interest by having either a small intron or a mutated internal restriction enzyme site. Therefore, the same primers were used to coamplify the unknown and the competitor. The ratio of products remains constant through the amplification and can be readily quantitated. In unstimulated cells, no GM-CSF or IL-3 mRNA could be detected. However, with appropriate induction, mRNA for both cytokines was detected and quantitated in as few as 200 cells. Competitive PCR was also used to accurately quantitate the copy number of the human GM-CSF gene in normal human cells, in a clonal population of cells from a patient with 5q- syndrome, and in a human-hamster cell line known to have only one copy of the human GM-CSF gene.
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Cites
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease
Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J., & Rutter, W. J. (1979) · Biochemistry · doi:10.1021/bi00591a005 · Cited by 21,725
Shaw, G., & Kamen, R. (1986) · Cell · doi:10.1016/0092-8674(86)90341-7 · Cited by 4,012
Higuchi, R., Krummel, B., & Saiki, R. (1988) · Nucleic Acids Research · doi:10.1093/nar/16.15.7351 · Cited by 2,387
Transcription of the dystrophin gene in human muscle and non-muscle tissues
Chelly, J., Kaplan, J.-C., Maire, P., Gautron, S., & Kahn, A. (1988) · Nature · doi:10.1038/333858a0 · Cited by 719
PCR Technology: Principles and Applications for DNA Amplification
Kellis, M. (1989) · Medical Entomology and Zoology · Cited by 688
Illegitimate transcription: transcription of any gene in any cell type.
Chelly, J., Concordet, J. P., Kaplan, J. C., & Kahn, A. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.8.2617 · Cited by 629
PCR Technology: Principles and Applications for DNA Amplification
Lynch, J. (1990) · Journal of Medical Genetics · doi:10.1136/jmg.27.8.536 · Cited by 505
Sarkar, G., & Sommer, S. S. (1989) · Science · doi:10.1126/science.2565599 · Cited by 304
Detection of amplified oncogenes by differential polymerase chain reaction.
Frye, R. A., Benz, C. C., & Liu, E. (1989) · PubMed · Cited by 206
Nienhuis, A. W., Franklin Bunn, H., Turner, P. H., Venkat Gopal, T., Nash, W. G., O'Brien, S. J., & Sherr, C. J. (1985) · Cell · doi:10.1016/0092-8674(85)90099-6 · Cited by 174
Cited by
Twenty-Five Years of Quantitative PCR for Gene Expression Analysis
VanGuilder, H. D., Vrana, K. E., & Freeman, W. M. (2008) · BioTechniques · doi:10.2144/000112776 · Cited by 1,246
Lifton, R. P., Dluhy, R. G., Powers, M., Rich, G. M., Cook, S., Ulick, S., & Lalouel, J.-M. (1992) · Nature · doi:10.1038/355262a0 · Cited by 1,158
Yoshioka, K., Kakumu, S., Wakita, T., Ishikawa, T., Itoh, Y., Takayanagi, M., Higashi, Y., Shibata, M., & Morishima, T. (1992) · Hepatology · doi:10.1002/hep.1840160203 · Cited by 668
Cloning and pharmacological characterization of a human bradykinin (BK-2) receptor
Hess, J. F., Borkowski, J. A., Young, G. S., Strader, C. D., & Ransom, R. W. (1992) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(92)91187-u · Cited by 470
Mizuno, S.-I., Chijiwa, T., Okamura, T., Akashi, K., Fukumaki, Y., Niho, Y., & Sasaki, H. (2001) · Blood · doi:10.1182/blood.v97.5.1172 · Cited by 438
Metadata
- Authors
- Gilliland, G., Perrin, S., Blanchard, K., & Bunn, H. F.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1990-04
- Volume
- 87
- Issue
- 7
- Pages
- 2725-2729
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2093009120
- Cited by
- 1,454
- References
- 13
- FWCI
- 25.39
- Type
- journal article
- Language
- en
- Open access
- green
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- Gilliland, G., Perrin, S., Blanchard, K., & Bunn, H. F. (1990). Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.87.7.2725
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