The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.
A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions
Open accessAbstract
Specific, end-labeled DNA fragments can be simply and rapidly prepared using the polymerase chain reaction (PCR). Such fragments are suitable for use in DNase I protection footprint assays, chemical sequencing reactions, and for the production and analysis of paused RNA polymerase transcription complexes. Moreover, a general means of introducing a specific mutation at any position along the length of such PCR-generated fragments is described. These procedures, which can circumvent the need for large-scale phage or plasmid growths, preparative gel-electrophoresis and the screening of molecular clones, can facilitate the rapid study of sequence-specific interactions of proteins and DNA. A rapid means of removing excess oligonucleotide primers from completed PCRs is also described.
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Cites
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Self-citation[21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction
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Self-citationDirect Cloning and Sequence Analysis of Enzymatically Amplified Genomic Sequences
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Cited by
Chinnaiyan, A. M., O'Rourke, K., Tewari, M., & Dixit, V. M. (1995) · Cell · doi:10.1016/0092-8674(95)90071-3 · Cited by 2,431
Tewari, M., Quan, L. T., O'Rourke, K., Desnoyers, S., Zeng, Z., Beidler, D. R., Poirier, G. G., Salvesen, G. S., & Dixit, V. M. (1995) · Cell · doi:10.1016/0092-8674(95)90541-3 · Cited by 2,367
Gilliland, G., Perrin, S., Blanchard, K., & Bunn, H. F. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.7.2725 · Cited by 1,454
Transcriptional activation and nuclear targeting signals of the human androgen receptor.
Simental, J. A., Sar, M., Lane, M. V., French, F. S., & Wilson, E. M. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)52466-2 · Cited by 631
Letourneur, F., & Klausner, R. D. (1992) · Cell · doi:10.1016/0092-8674(92)90636-q · Cited by 536
Metadata
- Authors
- Higuchi, R., Krummel, B., & Saiki, R.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1988
- Volume
- 16
- Issue
- 15
- Pages
- 7351-7367
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W1981765089
- Cited by
- 2,387
- References
- 16
- FWCI
- 17.41
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Higuchi, R., Krummel, B., & Saiki, R. (1988). A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions. Nucleic Acids Research. https://doi.org/10.1093/nar/16.15.7351
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
