The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
The second zinc-finger domain of poly(ADP-ribose) polymerase determines specificity for single-stranded breaks in DNA.
Open accessAbstract
Poly(ADP-ribose) polymerase (EC 2.4.2.30) is a zinc-binding protein that specifically binds to a DNA strand break in a zinc-dependent manner. We describe here the cloning and expression in Escherichia coli of a cDNA fragment encoding the two putative zinc fingers (FI and FII) domain of the human poly(ADP-ribose) polymerase. Using site-directed mutagenesis, we identified the amino acids involved in metal coordination and analyzed the consequence of altering the proposed zinc-finger structures on DNA binding. Disruption of the metal binding ability of the second zinc finger, FII, dramatically reduced target DNA binding. In contrast, when the postulated Zn(II) ligands of FI were mutated, the DNA binding activity was only slightly affected. DNase I protection studies showed that the FII is involved in the specific recognition of a DNA strand break. These results demonstrate that poly(ADP-ribose) polymerase contains a type of zinc finger that differs from previously recognized classes in terms of both structure and function.
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Cites
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Cantó, C., Menzies, K. J., & Auwerx, J. (2015) · Cell Metabolism · doi:10.1016/j.cmet.2015.05.023 · Cited by 1,648
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Self-citationPARP-2, A Novel Mammalian DNA Damage-dependent Poly(ADP-ribose) Polymerase
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Metadata
- Authors
- Gradwohl, G., Ménissier de Murcia, J. M., Molinete, M., Simonin, F., Koken, M., Hoeijmakers, J. H., & de Murcia, G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1990-04
- Volume
- 87
- Issue
- 8
- Pages
- 2990-2994
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2011476357
- Cited by
- 277
- References
- 24
- FWCI
- 6.48
- Type
- journal article
- Language
- en
- Open access
- green
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- Gradwohl, G., Ménissier de Murcia, J. M., Molinete, M., Simonin, F., Koken, M., Hoeijmakers, J. H., & de Murcia, G. (1990). The second zinc-finger domain of poly(ADP-ribose) polymerase determines specificity for single-stranded breaks in DNA. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.87.8.2990
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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