Molecular characterization of a protein-tyrosine-phosphatase enriched in striatum.
Open accessAbstract
A cDNA clone encoding a neural-specific putative protein-tyrosine-phosphatase (protein-tyrosine-phosphate phosphohydrolase, EC 3.1.3.48) has been isolated from a rat striatal cDNA library. The deduced amino acid sequence predicts a protein of approximately 369 amino acids with a strong homology to other members of the family of protein-tyrosine-phosphatases. In vitro translation produces a protein with an apparent molecular mass of 46 kDa. A potential attachment mechanism to the cytoplasmic membrane is suggested by a myristoylation amino acid-consensus sequence at the N terminus of the protein. RNA analyses of various regions of rat brain reveal a 3-kilobase (kb) and a 4.4-kb mRNA. The 3-kb mRNA is highly enriched within the striatum relative to other brain areas and has been termed a "striatum enriched phosphatase" (STEP). In contrast, the 4.4-kb message is most abundant in the cerebral cortex and rare in the striatum. These two messages appear to be alternatively processed RNA transcripts of a single gene.
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- Authors
- Lombroso, P. J., Murdoch, G., & Lerner, M.
- Affiliations
- Yale University (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1991-08-15
- Volume
- 88
- Issue
- 16
- Pages
- 7242-7246
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC52270
- OpenAlex
- W1992395108
- Cited by
- 188
- References
- 37
- Citations by year
- 2026: 3 · 2024: 5 · 2023: 4 · 2022: 1 · 2021: 4 · 2020: 2 · 2019: 3 · 2018: 4 · 2017: 5 · 2016: 10 · 2015: 6 · 2014: 10 · 2013: 5 · 2012: 22
- FWCI
- 3.70
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Howard Hughes Medical Institute
- National Institutes of Health (5k20mh000856-04)
- National Institute of Mental Health (MH00856-02)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Tyrosine Phosphatases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- protein tyrosine phosphatase, striatum, STEP, cDNA cloning, myristoylation, alternative splicing, mRNA isoforms, cerebral cortex, brain gene expression
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Brain, Cattle, Cloning, Molecular, Corpus Striatum, DNA, Molecular Sequence Data, Phosphoprotein Phosphatases, Putamen, RNA, RNA, Messenger, Transcription, Genetic, Protein Biosynthesis, Restriction Mapping, Gene Library, Protein Tyrosine Phosphatases, Rats
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