The DOI matches its Crossref record.
Pore-forming peptide of pathogenic Entamoeba histolytica.
Open accessAbstract
A polypeptide that causes pore formation in target-cell membranes is implicated in the potent cytolytic activity of pathogenic Entamoeba histolytica. Pore-forming material was purified to apparent homogeneity by a multistep procedure, and its analysis by NaDodSO4/PAGE revealed one peptide of 4-5 kDa under nonreducing or under reducing conditions. Pore-forming activity was measured by depolarization of liposome membrane potential and was found to be optimally expressed at low pH. Active material preferentially inserted into negatively charged lipid vesicles. Treatment of purified amoeba peptide in solution or bound to liposomes with glutaraldehyde revealed oligomers upon NaDodSO4/PAGE, suggesting functionally relevant peptide-peptide interactions. The NH2-terminal amino acid sequence of the amoeba peptide was determined by protein sequencing and revealed a structural similarity to melittin, the membranolytic peptide of bee venom.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Plank, C., Oberhauser, B., Mechtler, K., Koch, C., & Wagner, E. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)99963-1 · Cited by 666
Petri, W. A., Haque, R., & Mann, B. J. (2002) · Annual Review of Microbiology · doi:10.1146/annurev.micro.56.012302.160959 · Cited by 326
Lipid-binding Proteins in Membrane Digestion, Antigen Presentation, and Antimicrobial Defense
Kolter, T., Winau, F., Schaible, U. E., Leippe, M., & Sandhoff, K. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.r500015200 · Cited by 73
Self-citation
Metadata
- Authors
- Leippe, M., Ebel, S., Schoenberger, O. L., Horstmann, R. D., & Müller-Eberhard, H. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1991-09
- Volume
- 88
- Issue
- 17
- Pages
- 7659-7663
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2016035538
- Cited by
- 201
- References
- 0
- FWCI
- 4.41
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Pore-forming peptide of pathogenic Entamoeba histolytica. https://app.cochat.ai/papers/10.1073/pnas.88.17.7659
- The paper
- Leippe, M., Ebel, S., Schoenberger, O. L., Horstmann, R. D., & Müller-Eberhard, H. J. (1991). Pore-forming peptide of pathogenic Entamoeba histolytica. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.88.17.7659
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
