The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Eukaryotic DNA polymerase amino acid sequence required for 3'----5' exonuclease activity.
Open accessAbstract
We have identified an amino-proximal sequence motif, Phe-Asp-Ile-Glu-Thr, in Saccharomyces cerevisiae DNA polymerase II that is almost identical to a sequence comprising part of the 3'----5' exonuclease active site of Escherichia coli DNA polymerase I. Similar motifs were identified by amino acid sequence alignment in related, aphidicolin-sensitive DNA polymerases possessing 3'----5' proofreading exonuclease activity. Substitution of Ala for the Asp and Glu residues in the motif reduced the exonuclease activity of partially purified DNA polymerase II at least 100-fold while preserving the polymerase activity. Yeast strains expressing the exonuclease-deficient DNA polymerase II had on average about a 22-fold increase in spontaneous mutation rate, consistent with a presumed proofreading role in vivo. In multiple amino acid sequence alignments of this and two other conserved motifs described previously, five residues of the 3'----5' exonuclease active site of E. coli DNA polymerase I appeared to be invariant in aphidicolin-sensitive DNA polymerases known to possess 3'----5' proofreading exonuclease activity. None of these residues, however, appeared to be identifiable in the catalytic subunits of human, yeast, or Drosophila alpha DNA polymerases.
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Self-citationGenetic and Crystallographic Studies of the 3′,5′-Exonucleolytic Site of DNA Polymerase I
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Cited by
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Metadata
- Authors
- Morrison, A., Bell, J. B., Kunkel, T. A., & Sugino, A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1991-11
- Volume
- 88
- Issue
- 21
- Pages
- 9473-9477
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2047388928
- Cited by
- 276
- References
- 23
- FWCI
- 5.33
- Type
- journal article
- Language
- en
- Open access
- green
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- Morrison, A., Bell, J. B., Kunkel, T. A., & Sugino, A. (1991). Eukaryotic DNA polymerase amino acid sequence required for 3'----5' exonuclease activity. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.88.21.9473
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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