The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Cloning and expression of cDNA for a Na/Pi cotransport system of kidney cortex.
Open accessAbstract
A cDNA library from rabbit kidney cortex was screened for expression of Na-dependent transport of phosphate (Pi) using Xenopus laevis oocytes as an expression system. A single clone was eventually isolated (designated NaPi-1) that stimulated expression of Na/Pi cotransport approximately 700-fold compared to total mRNA. The predicted sequence of the Na/Pi cotransporter consists of 465 amino acids (relative molecular mass, 51,797); hydropathy profile predictions suggest six (possibly eight) membrane-spanning segments. In vitro translation of NaPi-1/complementary RNA in the presence of pancreatic microsomes indicated NaPi-1 to be a glycosylated protein; four potential N-glycosylation sites are present in the amino acid sequence. Northern blot analysis demonstrated the presence of NaPi-1/mRNA in kidney cortex and liver; no hybridization signal was obtained with mRNA from other tissues (including small intestine). Kinetic analysis of Na/Pi cotransport expressed by NaPi-1/complementary RNA demonstrated characteristics (sodium interaction) similar to those observed in cortical apical membranes. The alignment of 5 amino acid residues (Gly342/Ala381-Xaa-Xaa-Xaa-Xaa-Leu386-Xaa-Xaa-Xaa-P ro390- Arg391) is consistent with a motif proposed for Na-dependent transport systems. We conclude that we have cloned a cDNA for a Na/Pi cotransport system present in rabbit kidney cortex.
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Cites
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Cited by
Pao, S. S., Paulsen, I. T., & Saier, M. H. (1998) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.62.1.1-34.1998 · Cited by 2,297
Uptake of Glutamate into Synaptic Vesicles by an Inorganic Phosphate Transporter
Bellocchio, E. E., Reimer, R. J., Fremeau, R. T., & Edwards, R. H. (2000) · Science · doi:10.1126/science.289.5481.957 · Cited by 802
Kavanaugh, M. P., Miller, D. G., Zhang, W., Law, W., Kozak, S. L., Kabat, D., & Miller, A. D. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.15.7071 · Cited by 586
Varoqui, H., Schäfer, M. K.-H., Zhu, H., Weihe, E., & Erickson, J. D. (2002) · The Journal of Neuroscience · doi:10.1523/jneurosci.22-01-00142.2002 · Cited by 451
Expression cloning of human and rat renal cortex Na/Pi cotransport.
Magagnin, S., Werner, A., Markovich, D., Sorribas, V, Stange, G., Biber, J., & Murer, H. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.13.5979 · Cited by 351
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Metadata
- Authors
- Werner, A., Moore, M. L., Mantei, N., Biber, J., Semenza, G., & Murer, H.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1991-11
- Volume
- 88
- Issue
- 21
- Pages
- 9608-9612
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1975721317
- Cited by
- 265
- References
- 1
- FWCI
- 6.22
- Type
- journal article
- Language
- en
- Open access
- green
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- Werner, A., Moore, M. L., Mantei, N., Biber, J., Semenza, G., & Murer, H. (1991). Cloning and expression of cDNA for a Na/Pi cotransport system of kidney cortex. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.88.21.9608
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