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Cloning and functional expression of a vascular smooth muscle endothelin 1 receptor.
Open accessAbstract
By screening a cDNA library derived from the A10 rat vascular smooth muscle cell line for functional expression in COS cells, we have isolated a high-affinity receptor for endothelin 1 (Kd = 476 pM) and endothelin 2. The affinity of the cloned endothelin receptor for endothelin 3 is greater than 100 times less in A10 cells and in a CHO cell line stably transformed by the endothelin receptor cDNA. The 426-amino acid receptor polypeptide has seven putative hydrophobic transmembrane domains and is presumed to be a member of the family of guanine nucleotide-binding regulatory (G) protein-coupled receptors. Microinjection of in vitro transcripts of the cloned cDNA into CHO cells confers a transient increase in intracellular calcium in response to endothelin 1, indicating that the receptor is functional and couples to the appropriate G protein(s). RNA analysis reveals high expression in rat lung and heart, tissues known to exhibit binding to iodinated endothelin 1.
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Citations
Cited by
Thorens, B. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.18.8641 · Cited by 906
Expression cloning and characterization of the canine parietal cell gastrin receptor.
Kopin, A. S., Lee, Y. M., McBride, E. W., Miller, L. J., Lu, M., Lin, H. Y., Kolakowski, L. F., & Beinborn, M. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.8.3605 · Cited by 508
Self-citationExpression Cloning of an Adenylate Cyclase-Coupled Calcitonin Receptor
Lin, H. Y., Harris, T. L., Flannery, M. S., Aruffo, A., Kaji, E. H., Gorn, A., Kolakowski, L. F., Lodish, H. F., & Goldring, S. R. (1991) · Science · doi:10.1126/science.1658940 · Cited by 451
Self-citationChun, M., Liyanage, U. K., Lisanti, M. P., & Lodish, H. F. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.24.11728 · Cited by 334
Self-citationInactivation of endothelin I by deamidase (lysosomal protective protein).
Jackman, H. L., Morris, P. W., Deddish, P. A., Skidgel, R. A., & Erdös, E. G. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)50665-2 · Cited by 90
Metadata
- Authors
- Lin, H. Y., Kaji, E. H., Winkel, G. K., Ives, H. E., & Lodish, H. F.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1991-04-15
- Volume
- 88
- Issue
- 8
- Pages
- 3185-3189
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1979896022
- Cited by
- 352
- References
- 0
- FWCI
- 27.84
- Type
- journal article
- Language
- en
- Open access
- green
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- Lin, H. Y., Kaji, E. H., Winkel, G. K., Ives, H. E., & Lodish, H. F. (1991). Cloning and functional expression of a vascular smooth muscle endothelin 1 receptor. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.88.8.3185
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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