The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Proinsulin processing by the subtilisin-related proprotein convertases furin, PC2, and PC3.
Open accessAbstract
Experiments using recombinant vaccinia viruses expressing rat proinsulin I coinfected into COS-7 cells with recombinant vaccinia virus expressing human furin, human PC2, mouse PC3 (subtilisin-related proprotein convertases 1-3, respectively), or yeast Kex2 indicate that in this system both Kex2 and furin produce mature insulin, whereas PC2 selectively cleaves proinsulin at the C-peptide-A-chain junction. This is a property consistent with its probable identity with the rat insulinoma granule type II proinsulin processing activity as described by Davidson et al. [Davidson, H. W., Rhodes, C. J. & Hutton, J. C. (1988) Nature (London) 333, 93-96]. PC3 generates mature insulin but cleaves preferentially at the proinsulin B-chain-C-peptide junction. This pattern of cleavage by PC3 is similar, but not identical, to that of the highly B-chain-C-peptide junction-selective type I activity as described by Davidson et al., perhaps due to the presence of a P4 arginine residue near the C-peptide-A-chain junction unique to the rat proinsulins. These results along with data presented on the expression of both PC2 and PC3 in islet beta cells strongly support the conclusion that these proteases are involved in the conversion of proinsulin to insulin in vivo.
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Self-citationHosaka, M., Nagahama, M., Kim, W. S., Watanabe, T., Hatsuzawa, K., Ikemizu, J., Murakami, K., & Nakayama, K. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)98867-8 · Cited by 591
Post-Translational Proteolysis in Polypeptide Hormone Biosynthesis
Docherty, K., & Steiner, D. F. (1982) · Annual Review of Physiology · doi:10.1146/annurev.ph.44.030182.003205 · Cited by 590
Benjannet, S., Rondeau, N., Day, R., Chrétien, M., & Seidah, N. G. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.9.3564 · Cited by 589
Smeekens, S. P., & Steiner, D. F. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)39721-2 · Cited by 577
Self-citationSEIDAH, N. G., GASPAR, L., MION, P., MARCINKIEWICZ, M., MBIKAY, M., & CHRÉTIEN, M. (1990) · DNA and Cell Biology · doi:10.1089/dna.1990.9.415 · Cited by 512
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Cited by
The alphaviruses: gene expression, replication, and evolution.
Strauss, J. H., & Strauss, E. G. (1994) · Microbiological Reviews · doi:10.1128/mmbr.58.3.491-562.1994 · Cited by 2,221
Islet Amyloid Polypeptide, Islet Amyloid, and Diabetes Mellitus
Westermark, P., Andersson, A., & Westermark, G. T. (2011) · Physiological Reviews · doi:10.1152/physrev.00042.2009 · Cited by 1,044
The new enzymology of precursor processing endoproteases.
Steiner, D. F., Smeekens, S. P., Ohagi, S., & Chan, S. J. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)35852-6 · Cited by 613
Self-citationDefective prohormone processing and altered pancreatic islet morphology in mice lacking active SPC2
Furuta, M., Yano, H., Zhou, A., Rouillé, Y., Holst, J. J., Carroll, R., Ravazzola, M., Orci, L., Furuta, H., & Steiner, D. F. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.13.6646 · Cited by 431
Self-citationSeidah, N. G., Chrétien, M., & Day, R. (1994) · Biochimie · doi:10.1016/0300-9084(94)90147-3 · Cited by 396
Metadata
- Authors
- Smeekens, S. P., Montag, A. G., Thomas, G., Albiges-Rizo, C., Carroll, R., Benig, M., Phillips, L. A., Martin, S., Ohagi, S., & Gardner, P.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1992-09-15
- Volume
- 89
- Issue
- 18
- Pages
- 8822-8826
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2169293623
- Cited by
- 309
- References
- 36
- FWCI
- 28.12
- Type
- journal article
- Language
- en
- Open access
- green
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- Smeekens, S. P., Montag, A. G., Thomas, G., Albiges-Rizo, C., Carroll, R., Benig, M., Phillips, L. A., Martin, S., Ohagi, S., & Gardner, P. (1992). Proinsulin processing by the subtilisin-related proprotein convertases furin, PC2, and PC3. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.89.18.8822
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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