The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
DNA damage and mutation in human cells exposed to nitric oxide in vitro.
Open accessAbstract
Nitric oxide (NO.) is a physiological messenger formed by several cell types. Reaction with O2 forms oxides that nitrosate amines at pH values near 7. We now report experiments in which NO. was added to intact human cells and to aerobic solutions of DNA, RNA, guanine, or adenine. TK6 human lymphoblastoid cells were mutated 15- to 18-fold above background levels at both the HPRT and TK gene loci. Xanthine and hypoxanthine, from deamination of guanine and adenine, respectively, were formed in all cases. NO. induced dose-responsive DNA strand breakage. Yields of xanthine ranged from nearly equal to about 80-fold higher than those of hypoxanthine. Yields of xanthine and hypoxanthine from nucleic acids were higher than those from free guanine and adenine. This was most pronounced for xanthine; 0.3 nmol/mg was formed from free guanine vs. 550 nmol/mg from calf thymus RNA. Nitric oxide added to TK6 cells produced a 40- to 50-fold increase in hypoxanthine and xanthine in cellular DNA. We believe that these results, plus the expected deaminations of cytosine to uracil and 5-methylcytosine to thymine, account for the mutagenicity of nitric oxide toward bacteria and mammalian cells.
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Nitric oxide: physiology, pathophysiology, and pharmacology.
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Mutagenic deamination of cytosine residues in DNA
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Wink, D. A., & Mitchell, J. B. (1998) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(98)00092-6 · Cited by 1,606
Triggering and modulation of apoptosis by oxidative stress
Chandra, J., Samali, A., & Orrenius, S. (2000) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(00)00302-6 · Cited by 1,335
Nitric Oxide Activation of Poly(ADP-Ribose) Synthetase in Neurotoxicity
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Metadata
- Authors
- Nguyen, T., Brunson, D., Crespi, C. L., Penman, B. W., Wishnok, J. S., & Tannenbaum, S. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1992-04
- Volume
- 89
- Issue
- 7
- Pages
- 3030-3034
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2077641692
- Cited by
- 974
- References
- 4
- FWCI
- 24.86
- Type
- journal article
- Language
- en
- Open access
- green
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- Nguyen, T., Brunson, D., Crespi, C. L., Penman, B. W., Wishnok, J. S., & Tannenbaum, S. R. (1992). DNA damage and mutation in human cells exposed to nitric oxide in vitro. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.89.7.3030
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