Cited by 856Nitric Oxide and Endothelin EffectsHemoglobin structure and functionRedox biology and oxidative stress

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Oxidation of nitric oxide in aqueous solution to nitrite but not nitrate: comparison with enzymatically formed nitric oxide from L-arginine

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Ignarro, L J, Fukuto, J M, Griscavage, J M, Rogers, N E, Byrns, R E·1993·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.90.17.8103PMC PMC47296

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Abstract

Nitric oxide (NO) in oxygen-containing aqueous solution has a short half-life that is often attributed to a rapid oxidation to both NO2- and NO3-. The chemical fate of NO in aqueous solution is often assumed to be the same as that in air, where NO is oxidized to NO2 followed by dimerization to N2O4. Water then reacts with N2O4 to form both NO2- and NO3-. We report here that NO in aqueous solution containing oxygen is oxidized primarily to NO2- with little or no formation of NO3-. In the presence of oxyhemoglobin or oxymyoglobin, however, NO and NO2- were oxidized completely to NO3-. Methemoglobin was inactive in this regard. The unpurified cytosolic fraction from rat cerebellum, which contains constitutive NO synthase activity, catalyzed the conversion of L-arginine primarily to NO3- (NO2-/NO3- ratio = 0.25). After chromatography on DEAE-Sephacel or affinity chromatography using 2',5'-ADP-Sepharose 4B, active fractions containing NO synthase activity catalyzed the conversion of L-arginine primarily to NO2- (NO2-/NO3- ratio = 5.6) or only to NO2-, respectively. Unpurified cytosol from activated rat alveolar macrophages catalyzed the conversion of L-arginine to NO2- without formation of NO3-. Addition of 30 microM oxyhemoglobin to all enzyme reaction mixtures resulted in the formation primarily of NO3- (NO2-/NO3- ratio = 0.09 to 0.20). Cyanide ion, which displaces NO2- from its binding sites on oxyhemoglobin, inhibited the formation of NO3-, thereby allowing NO2- to accumulate. These observations indicate clearly that the primary decomposition product of NO in aerobic aqueous solution is NO2- and that further oxidation to NO3- requires the presence of additional oxidizing species such as oxyhemoproteins.

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Nitric oxide: physiology, pathophysiology, and pharmacology.
Moncada, S., Palmer, R. M., & Higgs, E. A. (1991) · Pharmacological Reviews · doi:10.1016/s0031-6997(25)06663-3 · Cited by 15,525
Nitric oxide as a secretory product of mammalian cells
Nathan, C. (1992) · The FASEB Journal · doi:10.1096/fasebj.6.12.1381691 · Cited by 4,350
Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme.
Bredt, D. S., & Snyder, S. H. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.2.682 · Cited by 3,308
Nitric oxide: A cytotoxic activated macrophage effector molecule
Hibbs, J. B., Taintor, R. R., Vavrin, Z., & Rachlin, E. M. (1988) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(88)80015-9 · Cited by 2,030
Macrophage oxidation of L-arginine to nitrite and nitrate: nitric oxide is an intermediate
Marletta, M. A., Yoon, P. S., Iyengar, R., Leaf, C. D., & Wishnok, J. S. (1988) · Biochemistry · doi:10.1021/bi00424a003 · Cited by 1,601
Biosynthesis And Metabolism Of Endothelium-Derived Nitric Oxide
Ignarro, L. J. (1990) · The Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pa.30.040190.002535 · Cited by 1,300
Endothelium-derived relaxing factor from pulmonary artery and vein possesses pharmacologic and chemical properties identical to those of nitric oxide radical.
Ignarro, L. J., Byrns, R. E., Buga, G. M., & Wood, K. S. (1987) · Circulation Research · doi:10.1161/01.res.61.6.866 · Cited by 1,164
Mammalian nitrate biosynthesis: mouse macrophages produce nitrite and nitrate in response to Escherichia coli lipopolysaccharide.
Stuehr, D. J., & Marletta, M. A. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.22.7738 · Cited by 1,126
Oxidation of nitrogen-oxides by bound dioxygen in hemoproteins
Doyle, M. P., & Hoekstra, J. W. (1981) · Journal of Inorganic Biochemistry · doi:10.1016/s0162-0134(00)80291-3 · Cited by 643
Nitric oxide synthase is a cytochrome P-450 type hemoprotein
White, K. A., & Marletta, M. A. (1992) · Biochemistry · doi:10.1021/bi00144a001 · Cited by 605

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Everett, S. A., Patel, K. B., Dennis, M. F., Smith, K. A., Stratford, M. R. L., & Wardman, P. (1998) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(97)00203-7 · Cited by 7
Nitric oxide generation from extracellularly applied NG-hydroxy-l-arginine in LPS-activated RAW 264 macrophages
Vetrovsky, P., Kleschyov, A. L., Entlicher, G., Poindron, P., & Stoclet, J. (1997) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/s0304-4165(96)00074-8 · Cited by 7
Nitric Oxide Affects Heme Oxygenase-1, Hepcidin, and Transferrin Receptor Expression in the Placenta
Principe, P., Mukosera, G. T., Gray-Hutto, N., Tugung, A., Gheorghe, C. P., & Blood, A. B. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24065887 · Cited by 6
“Sci. 2023, 24, x FOR PEER REVIEW 3 of 17 In aqueous buffer, free NO is oxidized predominantly into nitrite (NO2−), with a mi- nor fraction being further oxidized to nitrate (NO3−) [19].”
BackgroundInfluential
The Role of Nitric Oxide in Apoptosis and Autophagy
Loughran, P., Bagci, E. Z., Zamora, R., Yoram Vodovotz, B., & Billiar, T. R. (2010) · Elsevier eBooks · doi:10.1016/b978-0-12-373866-0.00015-0 · Cited by 6
Shear Stress and RBC-NOS Serine1177 Phosphorylation in Humans: A Dose Response
Horobin, J. T., Sabapathy, S., Kuck, L., & Simmonds, M. J. (2021) · Life · doi:10.3390/life11010036 · Cited by 6
The importance of and difficulties involved in creating molecular probes for a carbon monoxide gasotransmitter
Liu, H., Liu, T., Qin, Q., Li, B., Li, F., Zhang, B., & Sun, W. C. (2023) · The Analyst · doi:10.1039/d3an00849e · Cited by 6
Azotni oksid, neurodegeneracija i Parkinsonova bolest
Dzoljic, E., Nešić, I., Stojanović, M., Divac, N., Todorović, Z., Vuckovic, M., Kostic, S., & Prostran, S. M. (2005) · Vojnosanitetski pregled · doi:10.2298/vsp0510751d · Cited by 5
“Pored nNOS dodatni izvor NO u ekscitoksičnosti je iNOS u aktiviranoj mikrogliji (7, 41, 59, 60).”
Background
Ascorbic Acid Enhances Nitric Oxide Production in Trabecular Meshwork Cells
Kim, J. W. (2005) · Korean Journal of Ophthalmology · doi:10.3341/kjo.2005.19.3.227 · Cited by 6
The reaction of S-nitroso-N-acetyl-d,l-penicillamine (SNAP) with the angiotensin converting enzyme inhibitor, captopril—mechanism of transnitrosation
Aquart, D. V., & Dasgupta, T. P. (2005) · Organic & Biomolecular Chemistry · doi:10.1039/b500915d · Cited by 6
Skeletal Effects of Nitric Oxide
Wimalawansa, S. J. (2008) · Elsevier eBooks · doi:10.1016/b978-0-12-373884-4.00007-0 · Cited by 7
RETRACTED: A Potential Role of Apelin-13 against Hepatic Injury and Metabolic Disorders in Preeclampsia Induced by L-NAME
Hamza, R. Z., Diab, A. A. A., Zahra, M. H., Attia, M., Moursi, S. M. M., & Albaqami, N. M. (2021) · Coatings · doi:10.3390/coatings11040391 · Cited by 6
Retracted
Effects of different‐intensity laser acupuncture at two adjacent same‐meridian acupoints on nitric oxide and soluble guanylate cyclase releases in human
Jiang, W., Wei, H., Guo, Z., Ni, Y., Yang, H., & Xie, S. (2017) · Microcirculation · doi:10.1111/micc.12390 · Cited by 6
Are the Cavernous Tissue and Serum Levels of Micro RNAs 200a and 206 Elevated in Patients With Refractory Veno-occlusive Erectile Dysfunction? A Comparative Study
Din, S. F. G., Rashed, L. A., Alghobary, H. A. F., Tawfik, L. T., & ElSheemy, M. S. (2017) · Urology · doi:10.1016/j.urology.2017.07.020 · Cited by 6
Graphene in Electrochemical Biosensors
Lin, H.-Y., Chen, W., & Huang, C.-H. (2019) · Elsevier eBooks · doi:10.1016/b978-0-12-815889-0.00015-5 · Cited by 7
Randomised, double-blind, placebo-controlled clinical trial investigating the effects of inorganic nitrate in hypertension-induced target organ damage: protocol of the NITRATE-TOD study in the UK
Lau, C. W. Z., Hamers, A. J. P., Rathod, K. S., Shabbir, A., Cooper, J. A., Primus, C., Davies, C., Mathur, A., Moon, J., Kapil, V., et al. (2020) · BMJ Open · doi:10.1136/bmjopen-2019-034399 · Cited by 6
Diurnal variation in the proinflammatory activity of urban fine particulate matter (PM2.5) by in vitro assays
Lovett, C., Cacciottolo, M., Shirmohammadi, F., Haghani, A., Morgan, T. E., Sioutas, C., & Finch, C. E. (2018) · F1000Research · doi:10.12688/f1000research.14836.2 · Cited by 6
Rotating wall vessel as a newin vitro shear stress generation system: Application to rat coronary endothelial cell cultures
Morin, J.-P., Préterre, D., Keravec, V., & Thuillez, C. (2003) · Cell Biology and Toxicology · doi:10.1023/b:cbto.0000003844.59908.0e · Cited by 6
Studies on the insulinomimetic effects of benzylamine, exogenous substrate of semicarbazide-sensitive amine oxidase enzyme in streptozotocin induced diabetic rats
Soltész, Z., Tábi, T., Halász, A., Pálfi, M., Kocsis, E., Magyar, K., Tóth, M., & Szökő, É. (2007) · Journal of Neural Transmission · doi:10.1007/s00702-007-0700-1 · Cited by 6
“NO is transformed primarily to nitrite (Ignarro et al., 1993), while peroxynitrite and other RNS are converted directly to nitrate (van der Vliet et al.”
Background
Relationship between Serum Folate and Plasma Nitrate Concentrations: Possible Clinical Implications
Mansoor, M. A., Kristensen, O., Hervig, T. A., Stakkestad, J. A., Berge, T., Drabløs, P. A., Rolfsen, S., & Wentzel‐Larsen, T. (2005) · Clinical Chemistry · doi:10.1373/clinchem.2004.046409 · Cited by 6
Light‐Activated Transfer of Nitric Oxide from a Porous Material
Mitchell‐Koch, J. T., Reed, T. M., & Borovik, A. S. (2004) · Angewandte Chemie · doi:10.1002/ange.200352881 · Cited by 6
Nitrate, nitrite and nitric oxide in gastric mucosal defense.
Petersson, J. H. (2008) · Uppsala University Publications (Uppsala University) · Cited by 6
Regulation of Signal Transduction and Gene Expression by Reactive Nitrogen Species
Deora, A. A., & Lander, H. M. (2000) · Elsevier eBooks · doi:10.1016/b978-012636670-9/50009-x · Cited by 6
Activation and Regulation of the Nitric Oxide-Cyclic GMP Signal Transduction Pathway by Oxidative Stress
Ignarro, L. J. (1997) · Springer eBooks · doi:10.1007/978-1-4615-5981-8_1 · Cited by 6
Selective prolongation of NO donor activity of binuclear tetranitrosyl phenylmethanethiolate iron complexes upon binding to serum albumin
Luzhkov, V. B., Pokidova, O. V., Novikova, V. O., Mazina, L. M., Куликов, А. В., & Санина, Н. А. (2025) · Russian Chemical Bulletin · doi:10.1007/s11172-025-4561-2 · Cited by 5
Thio ligand binding in dinitrosyl iron-sulfur complexes: Quantitative structure–activity relationships
Luzhkov, V. B., Krapivin, V. B., Санина, Н. А., & Aldoshin, S. M. (2024) · Polyhedron · doi:10.1016/j.poly.2024.117013 · Cited by 5
Variability of PM measurement results due to subsampling of filters and water content analysis
Chyzhykov, D., Widziewicz-Rzońca, K., & Mathews, B. (2025) · Chemosphere · doi:10.1016/j.chemosphere.2025.144177 · Cited by 5
Transformation reactions of NO ligand in cationic nitrosyl iron complex [Fe(SC(NH2)2)2(NO)2]+ in aerobic aqueous solution: quantum chemical modeling and kinetic aspects
Emel’yanova, N. S., Gutsev, L. G., Pokidova, O. V., Shestakov, A. F., Санина, Н. А., & Aldoshin, S. M. (2024) · Russian Chemical Bulletin · doi:10.1007/s11172-024-4192-z · Cited by 5
Theoretical study of stability of dinitrosyl iron(i) complexes with thiocarbonyl ligands
Krapivin, V. B., Luzhkov, V. B., & Санина, Н. А. (2024) · Russian Chemical Bulletin · doi:10.1007/s11172-024-4435-z · Cited by 5
Nitric oxide affects immune cells bioenergetics
Hammami, I., Bertrand, M., Chen, J., Bronte, V., Crescenzo, G. D., & Jolicœur, M. (2012) · Immunobiology · doi:10.1016/j.imbio.2012.05.005 · Cited by 5
Optimization of the procedure of nitrogen oxide quantitation in mammalian cell culture media
Akimov, M., Fomina-Ageeva, E. V., & Безуглов, В. В. (2015) · Russian Journal of Bioorganic Chemistry · doi:10.1134/s1068162015010021 · Cited by 5
Serum nitric oxide levels and flow-mediated dilatation in patients with Sheehan syndrome and the effect of combination therapy consisting of L-thyroxine, prednisolone, and conjugated estrogen/medroxyprogesterone acetate
Bahçeci, M., Paşa, S., Akay, H., Tuzcu, A., Tümer, C., & Gökalp, D. (2007) · Fertility and Sterility · doi:10.1016/j.fertnstert.2007.04.014 · Cited by 5
Oxidation of NO˙ by small oxygen species HO 2 − and O 2 ˙ − : the role of negative charge, electronic spin and water solvation
Ryding, M. J., Fernández, I., & Uggerud, E. (2016) · Physical Chemistry Chemical Physics · doi:10.1039/c6cp00290k · Cited by 5
High-Resolution Capillary Electrophoresis of Nitrite and Nitrate in Biological Samples
Boudko, D. Y. (2004) · Humana Press eBooks · doi:10.1385/1-59259-807-2:009 · Cited by 5
High-protein nutrition during pregnancy increases neuroinflammation and homocysteine levels and impairs behavior in male adolescent rats offspring
Silveira, J. S., Júnior, O. V. R., Schmitz, F., Ferreira, F. S., Rodrigues, F. C., Deon, M., Ribas, G. S., Coutinho‐Silva, R., Vargas, C. R., Savio, L. E. B., et al. (2022) · Life Sciences · doi:10.1016/j.lfs.2022.121084 · Cited by 5
Nitric Oxide Expression in Cancer
Masini, E., Cianchi, F., Mastroianni, R., & Cuzzocrea, S. (2010) · Springer eBooks · doi:10.1007/978-1-4419-1432-3_4 · Cited by 5
“, oxyhemoglobin) and the stable end-product that can be measured in plasma is nitrate (Butler and Williams 1993; Feldman et al. 1993; Ignarro et al. 1993).”
Background
Optical and biological properties of plasma-treated Neurospora crassa spores as studied by absorption, circular dichroism, and Raman spectroscopy
Lee, G. J., Park, G., & Choi, E. H. (2017) · Journal of the Korean Physical Society · doi:10.3938/jkps.71.670 · Cited by 5

Authors: Ignarro, L. J., Fukuto, J. M., Griscavage, J. M., Rogers, N. E., & Byrns, R. E.
Affiliations: University of California, Los Angeles (US)
ORCID: Fukuto, J M
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 1993-09
Volume: 90
Issue: 17
Pages: 8103-8107
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.90.17.8103
PMC: PMC47296
OpenAlex: W2033379487
Cited by: 856
References: 28
Citations by year: 2026: 14 · 2025: 14 · 2024: 19 · 2023: 21 · 2022: 14 · 2021: 15 · 2020: 21 · 2019: 19 · 2018: 20 · 2017: 21 · 2016: 18 · 2015: 23 · 2014: 28 · 2013: 27 · 2012: 24
FWCI: 17.78
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health (5r01hl040922-11, 5r01hl046388-03, 5r01hl035014-12)
National Heart, Lung, and Blood Institute (HL 46388, HL-35014, HL 40922)
Topics: Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Hemoglobin structure and function
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Keywords: NO oxidation, nitrite, nitrate, aqueous solutions, oxyhemoglobin, oxymyoglobin, methemoglobin, nitric oxide synthase, L-arginine, alveolar macrophages, cyanide, heme proteins
MeSH terms: Amino Acid Oxidoreductases, Animals, Arginine, Hemeproteins, Humans, Kinetics, Methemoglobin, Myoglobin, Nitrates, Nitric Oxide, Nitrites, Oxidation-Reduction, Oxyhemoglobins, Solutions, Nitric Oxide Synthase

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Oxidation of nitric oxide in aqueous solution to nitrite but not nitrate: comparison with enzymatically formed nitric oxide from L-arginine

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