The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Activation by nitric oxide of an oxidative-stress response that defends Escherichia coli against activated macrophages.
Open accessAbstract
Nitric oxide is a free radical (NO) formed biologically through the oxidation of L-arginine by nitric oxide synthases. NO is produced transiently in mammalian cells for intercellular signaling and in copious quantities to cause cytostasis and cytotoxicity. In the latter situation, NO is a deliberate cytotoxic product of activated macrophages, along with other reactive oxygen species such as hydrogen peroxide (H2O2) and superoxide (O2-). Escherichia coli has a complex set of responses to H2O2 and O2- that involves approximately 80 inducible proteins; we wondered whether these bacteria might induce analogous defenses against nitric oxide. We show here that a multigene system controlled by the redox-sensitive transcriptional regulator SoxR is activated by NO in vivo. This induction confers bacterial resistance to activated murine macrophages with kinetics that parallel the production of NO by these cells. Elimination of specific SoxR-regulated genes diminishes the resistance of these bacteria to the cytotoxic macrophages. The required functions include manganese-containing superoxide dismutase, endonuclease IV (a DNA-repair enzyme for oxidative damage), and micF, an antisense regulator of the outer membrane porin OmpF. These results demonstrate that SoxR is a sensor for cellular exposure to NO, and that the soxRS response system may contribute to bacterial virulence.
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Cites
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Peroxynitrite, a cloaked oxidant formed by nitric oxide and superoxide
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Cited by
Redox signaling: Nitrosylation and related target interactions of nitric oxide
Stamler, J. S. (1994) · Cell · doi:10.1016/0092-8674(94)90269-0 · Cited by 1,723
Wink, D. A., & Mitchell, J. B. (1998) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(98)00092-6 · Cited by 1,606
The chemistry of peroxynitrite: A product from the reaction of nitric oxide with superoxide
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Stamler (2001) · doi:10.1016/s0092-8674(01)00495-0 · Cited by 1,261
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Metadata
- Authors
- Nunoshiba, T., deRojas-Walker, T., Wishnok, J. S., Tannenbaum, S. R., & Demple, B.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1993-11
- Volume
- 90
- Issue
- 21
- Pages
- 9993-9997
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2001207916
- Cited by
- 310
- References
- 38
- FWCI
- 8.29
- Type
- journal article
- Language
- en
- Open access
- green
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- Nunoshiba, T., deRojas-Walker, T., Wishnok, J. S., Tannenbaum, S. R., & Demple, B. (1993). Activation by nitric oxide of an oxidative-stress response that defends Escherichia coli against activated macrophages. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.90.21.9993
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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