The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Molecular cloning and expression of inducible nitric oxide synthase from human hepatocytes.
Open accessAbstract
Nitric oxide is a short-lived biologic mediator for diverse cell types. Synthesis of an inducible nitric oxide synthase (NOS) in murine macrophages is stimulated by lipopolysaccharide (LPS) and interferon gamma. In human hepatocytes, NOS activity is induced by treatment with a combination of tumor necrosis factor, interleukin 1, interferon gamma, and LPS. We now report the molecular cloning and expression of an inducible human hepatocyte NOS (hep-NOS) cDNA. hep-NOS has 80% amino acid sequence homology to macrophage NOS (mac-NOS). Like other NOS isoforms, recognition sites for FMN, FAD, and NADPH are present, as well as a consensus calmodulin binding site. NOS activity in human 293 kidney cells transfected with hep-NOS cDNA is diminished by Ca2+ chelation and a calmodulin antagonist, reflecting a Ca2+ dependence not evident for mac-NOS. Northern blot analysis with hep-NOS cDNA reveals a 4.5-kb mRNA in both human hepatocytes and aortic smooth muscle cells following stimulation with LPS and cytokines. Human genomic Southern blots probed with human hep-NOS and human endothelial NOS cDNA clones display different genomic restriction enzyme fragments, suggesting distinct gene products for these NOS isoforms. hep-NOS appears to be an inducible form of NOS that is distinct from mac-NOS as well as brain and endothelial NOS isozymes.
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Cites
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Stimulation of the nitric oxide synthase pathway in human hepatocytes by cytokines and endotoxin
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Cited by
Nitric oxide synthases: structure, function and inhibition
ALDERTON, W. K., COOPER, C. E., & KNOWLES, R. G. (2001) · Biochemical Journal · doi:10.1042/bj3570593 · Cited by 4,110
Nitric oxide synthases in mammals
Knowles, R. G., & Moncada, S. (1994) · Biochemical Journal · doi:10.1042/bj2980249 · Cited by 2,752
Gases as biological messengers: nitric oxide and carbon monoxide in the brain
Dawson, T., & Snyder, S. (1994) · The Journal of Neuroscience · doi:10.1523/jneurosci.14-09-05147.1994 · Cited by 1,043
Self-citationNitric oxide synthase: Aspects concerning structure and catalysis
Marletta, M. A. (1994) · Cell · doi:10.1016/0092-8674(94)90268-2 · Cited by 854
Induction of nitric oxide synthase in asthma
Hamid, Q., Springall, D., Polak, J., Riveros-Moreno, V, Chanez, P., Bousquet, J., Godard, P., Holgate, S., Howarth, P., & Redington, A. (1993) · The Lancet · doi:10.1016/s0140-6736(05)80083-2 · Cited by 836
Metadata
- Authors
- Geller, D. A., Lowenstein, C. J., Shapiro, R. A., Nussler, A. K., Di Silvio, M., Wang, S. C., Nakayama, D. K., Simmons, R. L., Snyder, S. H., & Billiar, T. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1993-04-15
- Volume
- 90
- Issue
- 8
- Pages
- 3491-3495
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2016687652
- Cited by
- 821
- References
- 7
- FWCI
- 51.39
- Type
- journal article
- Language
- en
- Open access
- green
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- Geller, D. A., Lowenstein, C. J., Shapiro, R. A., Nussler, A. K., Di Silvio, M., Wang, S. C., Nakayama, D. K., Simmons, R. L., Snyder, S. H., & Billiar, T. R. (1993). Molecular cloning and expression of inducible nitric oxide synthase from human hepatocytes. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.90.8.3491
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