The DOI matches its Crossref record.
Structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes.
Open accessAbstract
In Pseudomonas aeruginosa the LasR protein is required for activation of lasB and several other virulence genes. A diffusible signal molecule, the P. aeruginosa autoinducer (PAI), produced by the bacterial cell and released into the growth medium, is required for activity of LasR. By cloning a lasB::lacZ fusion and a lasR gene under control of the lac promoter in Escherichia coli, we have developed a quantitative bioassay for PAI. We have used this assay to follow the purification of PAI from cell-free culture supernatant fluids in which P. aeruginosa or E. coli containing the P. aeruginosa gene required for autoinducer synthesis, lasI, had been grown. Chemical analyses indicated the purified material was 3-oxo-N-(tetrahydro-2-oxo-3-furanyl)dodecanamide. To confirm this assignment, the compound was synthesized and the synthetic compound was shown to have chemical and biological properties identical to those of PAI purified from culture supernatant fluids. The elucidation of the PAI structure suggests therapeutic approaches toward control of P. aeruginosa infections.
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Cited by
The Involvement of Cell-to-Cell Signals in the Development of a Bacterial Biofilm
Davies, D. G., Parsek, M. R., Pearson, J. P., Iglewski, B. H., Costerton, J. W., & Greenberg, E. P. (1998) · Science · doi:10.1126/science.280.5361.295 · Cited by 3,396
Self-citationPseudomonas aeruginosa Displays Multiple Phenotypes during Development as a Biofilm
Sauer, K., Camper, A. K., Ehrlich, G. D., Costerton, J. W., & Davies, D. G. (2002) · Journal of Bacteriology · doi:10.1128/jb.184.4.1140-1154.2002 · Cited by 1,691
Genetic adaptation by Pseudomonas aeruginosa to the airways of cystic fibrosis patients
Smith, E. E., Buckley, D. G., Wu, Z., Saenphimmachak, C., Hoffman, L. R., D’Argenio, D. A., Miller, S. I., Ramsey, B. W., Speert, D. P., Moskowitz, S. M., et al. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0602138103 · Cited by 1,319
A Pseudomonas aeruginosa quorum‐sensing molecule influences Candida albicans morphology
Hogan, D. A., Vik, Å., & Kolter, R. (2004) · Molecular Microbiology · doi:10.1111/j.1365-2958.2004.04349.x · Cited by 575
Social cheating in Pseudomonas aeruginosa quorum sensing
Sandoz, K. M., Mitzimberg, S. M., & Schuster, M. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0705653104 · Cited by 480
Metadata
- Authors
- Pearson, J. P., Gray, K. M., Passador, L., Tucker, K. D., Eberhard, A., Iglewski, B. H., & Greenberg, E. P.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1994-01-04
- Volume
- 91
- Issue
- 1
- Pages
- 197-201
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1977105094
- Cited by
- 990
- References
- 0
- FWCI
- 7.35
- Type
- journal article
- Language
- en
- Open access
- green
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- Pearson, J. P., Gray, K. M., Passador, L., Tucker, K. D., Eberhard, A., Iglewski, B. H., & Greenberg, E. P. (1994). Structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.91.1.197
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