Rch1, a protein that specifically interacts with the RAG-1 recombination-activating protein
Open accessAbstract
RAG1 and RAG2 are lymphoid-specific genes that together induce V(D)J recombinase activity in a variety of nonlymphoid cell types. While no other lymphoid-specific factors are required to induce recombination, other factors with more widespread expression patterns have been implicated in the reaction. However, none of these factors have been cloned, and their relationship to the RAG proteins is unclear. Using the yeast two-hybrid assay, we have identified RCH1, a gene encoding a protein of molecular weight 58,000 that interacts specifically with RAG-1. The predicted Rch1 protein sequence is 47% identical to yeast SRP1, a protein associated with the nuclear envelope. A truncated form of Rch1, which retains the ability to interact with RAG-1, reduces V(D)J recombination activity in HeLa cells.
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In humans, six importin-α isoforms are known.
“In humans, six importin-α isoforms are known [41], [42], [43], [44].”
From UV-Induced Nuclear Import of XPA Is Mediated by Importin-α4 in An ATR-Dependent Manner (2013) · cites this paper as [42]
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Background
- Authors
- Cuomo, C. A., Kirch, S. A., Gyuris, J., Brent, R., & Oettinger, M. A.
- Affiliations
- Harvard University (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1994-06-21
- Volume
- 91
- Issue
- 13
- Pages
- 6156-6160
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC44157
- OpenAlex
- W2150627415
- Cited by
- 164
- References
- 28
- Citations by year
- 2022: 1 · 2021: 1 · 2020: 1 · 2016: 1 · 2015: 4 · 2014: 3 · 2013: 4 · 2012: 5
- FWCI
- 7.86
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of General Medical Sciences (GM 48026, R01GM048026)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Keywords
- RAG1, RAG2, V(D)J recombination, protein-protein interaction, nuclear envelope, HeLa cells
- MeSH terms
- Amino Acid Sequence, B-Lymphocytes, Base Sequence, Carrier Proteins, Cell Line, Chromosome Mapping, Chromosomes, Human, Pair 17, DNA Nucleotidyltransferases, Enzyme Induction, HeLa Cells, Humans, Molecular Sequence Data, Molecular Weight, Nuclear Proteins, Organ Specificity, Proteins, Recombinant Fusion Proteins, Repetitive Sequences, Nucleic Acid, Saccharomyces cerevisiae, T-Lymphocytes, Protein Biosynthesis, Restriction Mapping, Gene Expression, Sequence Homology, Amino Acid, Homeodomain Proteins, alpha Karyopherins, VDJ Recombinases
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