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The pleckstrin homology domain of Bruton tyrosine kinase interacts with protein kinase C.
Open accessAbstract
Bruton tyrosine kinase (EC 2.7.1.112) [Btk, encoded by Btk in mice and BTK in humans (formerly known as atk, BPK, or emb)], which is variously mutated in chromosome X-linked agammaglobulinemia patients and X-linked immunodeficient (xid) mice, has the pleckstrin homology (PH) domain at its amino terminus. The PH domain of Btk expressed as a bacterial fusion protein directly interacts with protein kinase C in mast cell lysates. Evidence was obtained that Btk is physically associated with protein kinase C in intact murine mast cells as well. Both Ca(2+)-dependent (alpha, beta I, and beta II) and Ca(2+)-independent protein kinase C isoforms (epsilon and zeta) in mast cells interact with the PH domain of Btk in vitro, and protein kinase C beta I is associated with Btk in vivo. Btk served as a substrate of protein kinase C, and its enzymatic activity was down-regulated by protein kinase C-mediated phosphorylation. Furthermore, depletion or inhibition of protein kinase C with pharmacological agents resulted in an enhancement of the tyrosine phosphorylation of Btk induced by mast cell activation.
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Cited by
Protein modules and signalling networks
Pawson, T. (1995) · Nature · doi:10.1038/373573a0 · Cited by 2,420
Specific and high-affinity binding of inositol phosphates to an isolated pleckstrin homology domain
Lemmon, M. A., Ferguson, K. M., O'Brien, R., Sigler, P. B., & Schlessinger, J. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.23.10472 · Cited by 566
Specificity and promiscuity in phosphoinositide binding by pleckstrin homology domains
Kavran, J. M., Klein, D. E., Lee, A., Falasca, M., Isakoff, S. J., Skolnik, E. Y., & Lemmon, M. A. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.46.30497 · Cited by 434
Scharenberg, A. M., El-Hillal, O., Fruman, D. A., Beitz, L. O., Li, Z., Lin, S., Gout, I., Cantley, L. C., Rawlings, D. J., & Kinet, J.-P. (1998) · The EMBO Journal · doi:10.1093/emboj/17.7.1961 · Cited by 429
The tyrosine kinase network regulating mast cell activation
Gilfillan, A. M., & Rivera, J. (2009) · Immunological Reviews · doi:10.1111/j.1600-065x.2008.00742.x · Cited by 428
Metadata
- Authors
- Yao, L., Kawakami, Y., & Kawakami, T.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1994-09-13
- Volume
- 91
- Issue
- 19
- Pages
- 9175-9179
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2041121472
- Cited by
- 316
- References
- 0
- FWCI
- 14.49
- Type
- journal article
- Language
- en
- Open access
- green
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- Yao, L., Kawakami, Y., & Kawakami, T. (1994). The pleckstrin homology domain of Bruton tyrosine kinase interacts with protein kinase C. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.91.19.9175
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