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Efficient gene transfer into human hepatocytes by baculovirus vectors.
Open accessAbstract
Viral vectors are the most efficient tools for gene delivery, and the search for tissue-specific infecting viruses is important for the development of in vivo gene therapy strategies. The baculovirus Autographa californica nuclear polyhedrosis virus is widely used as a vector for expression of foreign genes in insect cells, and its host specificity is supposed to be restricted to arthropods. Here we demonstrate that recombinant A. californica nuclear polyhedrosis virus is efficiently taken up by human hepatocytes via an endosomal pathway. High-level reporter gene expression from heterologous promoters was observed in human and rabbit hepatocytes in vitro. Mouse hepatocytes and some other epithelial cell types are targeted at a considerably lower rate. The efficiency of gene transfer by baculovirus considerably exceeds that obtained by calcium phosphate or lipid transfection. These properties of baculovirus suggest a use for it as a vector for liver-directed gene transfer but highlight a potential risk in handling certain recombinant baculoviruses.
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Cites
Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.
Boukamp, P., Petrussevska, R. T., Breitkreutz, D., Hornung, J., Markham, A., & Fusenig, N. E. (1988) · The Journal of cell biology · doi:10.1083/jcb.106.3.761 · Cited by 4,170
Cited by
Baculovirus as versatile vectors for protein expression in insect and mammalian cells
Kost, T. A., Condreay, J. P., & Jarvis, D. L. (2005) · Nature Biotechnology · doi:10.1038/nbt1095 · Cited by 1,014
Condreay, J. P., Witherspoon, S. M., Clay, W. C., & Kost, T. A. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.1.127 · Cited by 420
Expression of Recombinant Antibodies
Frenzel, A., Hust, M., & Schirrmann, T. (2013) · Frontiers in Immunology · doi:10.3389/fimmu.2013.00217 · Cited by 353
(BIOHAZ), E. P. O. B. H. (2013) · EFSA Journal · doi:10.2903/j.efsa.2013.3449 · Cited by 328
New baculovirus expression tools for recombinant protein complex production
Trowitzsch, S., Bieniossek, C., Nie, Y., Garzoni, F., & Berger, I. (2010) · Journal of Structural Biology · doi:10.1016/j.jsb.2010.02.010 · Cited by 224
Metadata
- Authors
- Hofmann, C., Sandig, V, Jennings, G., Rudolph, M., Schlag, P., & Strauss, M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1995-10-24
- Volume
- 92
- Issue
- 22
- Pages
- 10099-10103
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2076003033
- Cited by
- 492
- References
- 1
- FWCI
- 3.43
- Type
- journal article
- Language
- en
- Open access
- green
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- Hofmann, C., Sandig, V, Jennings, G., Rudolph, M., Schlag, P., & Strauss, M. (1995). Efficient gene transfer into human hepatocytes by baculovirus vectors. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.92.22.10099
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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