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Cited by 1,014Viral Infectious Diseases and Gene Expression in InsectsTransgenic Plants and ApplicationsInsect Utilization and Effects

Baculovirus as versatile vectors for protein expression in insect and mammalian cells

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Kost, Thomas A, Condreay, J Patrick, Jarvis, Donald L·2005·Nature Biotechnology
DOI 10.1038/nbt1095PMC PMC3610534
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Abstract

Today, many thousands of recombinant proteins, ranging from cytosolic enzymes to membrane-bound proteins, have been successfully produced in baculovirus-infected insect cells. Yet, in addition to its value in producing recombinant proteins in insect cells and larvae, this viral vector system continues to evolve in new and unexpected ways. This is exemplified by the development of engineered insect cell lines to mimic mammalian cell glycosylation of expressed proteins, baculovirus display strategies and the application of the virus as a mammalian-cell gene delivery vector. Novel vector design and cell engineering approaches will serve to further enhance the value of baculovirus technology.

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  • Insect cells produce N-glycan precursors that are trimmed, creating high mannose or paucimannose structures.

    “Insect cells produce N-glycan precursors that are trimmed, creating high mannose or paucimannose structures (Kost et al., 2005).”

    From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Kost et al., 2005

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Cited by

Sorted by citations

Authors
Kost, T. A., Condreay, J. P., & Jarvis, D. L.
Affiliations
  • GlaxoSmithKline (United States) (US)
  • University of Wyoming (US)
Corresponding author
Kost, Thomas A
ORCID
Jarvis, Donald L
Venue
Nature Biotechnology
Publisher
Springer Science and Business Media LLC
Published
2005-05-01
Volume
23
Issue
5
Pages
567-575
ISSN
  • 0733-222X (ISSN-L)
  • 1087-0156
  • 1546-1696
DOI
10.1038/nbt1095
PMC
PMC3610534
OpenAlex
W2075470662
Cited by
1,014
References
0
Citations by year
2026: 31 · 2025: 30 · 2024: 35 · 2023: 18 · 2022: 35 · 2021: 34 · 2020: 32 · 2019: 35 · 2018: 37 · 2017: 46 · 2016: 45 · 2015: 44 · 2014: 59 · 2013: 68 · 2012: 53
FWCI
5.05
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Biotechnology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Science Foundation (9818001, 9814157)
  • GlaxoSmithKline
  • National Institutes of Health
  • National Institute of General Medical Sciences (GM 49734, R01GM049734)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect Utilization and Effects
Keywords
baculovirus, recombinant protein expression, insect cell expression, insect cell lines, glycosylation, gene delivery, vector design, membrane proteins
MeSH terms
Animals, Biotechnology, Genetic Vectors, Humans, Insecta, Mammals, Recombinant Proteins, Transfection, Protein Engineering, Baculoviridae, Genetic Enhancement

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