The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Processing and cryopreservation of placental/umbilical cord blood for unrelated bone marrow reconstitution.
Open accessAbstract
Clinical evidence of hematopoietic restoration with placental/umbilical cord blood (PCB) grafts indicates that PCB can be a useful source of hematopoietic stem cells for routine bone marrow reconstitution. In the unrelated setting, human leukocyte antigen (HLA)-matched donors must be obtained for candidate patients and, hence, large panels of frozen HLA-typed PCB units must be established. The large volume of unprocessed units, consisting mostly of red blood cells, plasma, and cryopreservation medium, poses a serious difficulty in this effort because storage space in liquid nitrogen is limited and costly. We report here that almost all the hematopoietic colony-forming cells present in PCB units can be recovered in a uniform volume of 20 ml by using rouleaux formation induced by hydroxyethyl starch and centrifugation to reduce the bulk of erythrocytes and plasma and, thus, concentrate leukocytes. This method multiples the number of units that can be stored in the same freezer space as much as 10-fold depending on the format of the storage system. We have also investigated the proportion of functional stem/progenitor cells initially present that are actually available to the recipient when thawed cryopreserved PCB units are infused. Progenitor cell viability is measurably decreased when thawed cells, still suspended in hypertonic cryopreservative solutions, are rapidly mixed with large volumes of isotonic solutions or plasma. The osmotic damage inflicted by the severe solute concentration gradient, however, can be averted by a simple 2-fold dilution after thawing, providing almost total recovery of viable hematopoietic progenitor cells.
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Self-citationStored placental blood for unrelated bone marrow reconstitution
Rubinstein, P., Rosenfield, R., Adamson, J., & Stevens, C. (1993) · Blood · doi:10.1182/blood.v81.7.1679.1679 · Cited by 405
Self-citationOsmotic stress as a mechanism of freezing injury
Meryman, H. T. (1971) · Cryobiology · doi:10.1016/0011-2240(71)90040-x · Cited by 236
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Adverse reactions in patients transfused with cryopreserved marrow
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Collection, separation and cryopreservation of umbilical cord blood for use in transplantation.
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Cited by
Cord-Blood Transplants from Unrelated Donors in Patients with Hurler's Syndrome
Staba, S. L., Escolar, M. L., Poe, M., Kim, Y., Martin, P. L., Szabolcs, P., Allison-Thacker, J., Wood, S., Wenger, D. A., Rubinstein, P., et al. (2004) · New England Journal of Medicine · doi:10.1056/nejmoa032613 · Cited by 478
Self-citationCryopreservation of Human Stem Cells for Clinical Application: A Review
Hunt, C. J. (2011) · Transfusion Medicine and Hemotherapy · doi:10.1159/000326623 · Cited by 329
Locatelli, F., Kabbara, N., Ruggeri, A., Ghavamzadeh, A., Roberts, I., Li, C. K., Bernaudin, F., Vermylen, C., Dalle, J.-H., Stein, J., et al. (2013) · Blood · doi:10.1182/blood-2013-03-489112 · Cited by 244
De Felice, L., Tatarelli, C., Mascolo, M. G., Gregorj, C., Agostini, F., Fiorini, R., Gelmetti, V., Pascale, S., Padula, F., Petrucci, M. T., et al. (2005) · Cancer Research · doi:10.1158/0008-5472.can-04-3063 · Cited by 149
Outcomes of Unrelated Umbilical Cord Blood Transplantation for X-Linked Adrenoleukodystrophy
Beam, D., Poe, M. D., Provenzale, J. M., Szabolcs, P., Martin, P. L., Prasad, V., Parikh, S., Driscoll, T., Mukundan, S., Kurtzberg, J., et al. (2007) · Biology of Blood and Marrow Transplantation · doi:10.1016/j.bbmt.2007.01.082 · Cited by 83
Metadata
- Authors
- Rubinstein, P., Dobrila, L., Rosenfield, R. E., Adamson, J. W., Migliaccio, G., Migliaccio, A. R., Taylor, P. E., & Stevens, C. E.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1995-10-24
- Volume
- 92
- Issue
- 22
- Pages
- 10119-10122
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2010055592
- Cited by
- 819
- References
- 8
- FWCI
- 10.90
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Processing and cryopreservation of placental/umbilical cord blood for unrelated bone marrow reconstitution. https://app.cochat.ai/papers/10.1073/pnas.92.22.10119
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- Rubinstein, P., Dobrila, L., Rosenfield, R. E., Adamson, J. W., Migliaccio, G., Migliaccio, A. R., Taylor, P. E., & Stevens, C. E. (1995). Processing and cryopreservation of placental/umbilical cord blood for unrelated bone marrow reconstitution. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.92.22.10119
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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