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Production of infectious human respiratory syncytial virus from cloned cDNA confirms an essential role for the transcription elongation factor from the 5' proximal open reading frame of the M2 mRNA in gene expression and provides a capability for vaccine development.
Open accessAbstract
Infectious human respiratory syncytial virus (RSV) was produced by the intracellular coexpression of five plasmid-borne cDNAs. One cDNA encoded a complete positive-sense version of the RSV genome (corresponding to the replicative intermediate RNA or antigenome), and each of the other four encoded a separate RSV protein, namely, the major nucleocapsid N protein, the nucleocapsid P phosphoprotein, the major polymerase L protein, or the protein from the 5' proximal open reading frame of the M2 mRNA [M2(ORF1)]. RSV was not produced if any of the five plasmids was omitted. The requirement for the M2(ORF1) protein is consistent with its recent identification as a transcription elongation factor and confirms its importance for RSV gene expression. It should thus be possible to introduce defined changes into infectious RSV. This should be useful for basic studies of RSV molecular biology and pathogenesis; in addition, there are immediate applications to the development of live attenuated vaccine strains bearing predetermined defined attenuating mutations.
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Citations
Cited by
BASIC MECHANISMS OF TRANSCRIPT ELONGATION AND ITS REGULATION
Uptain, S. M., Kane, C. M., & Chamberlin, M. J. (1997) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.66.1.117 · Cited by 445
Karron, R. A., Buonagurio, D. A., Georgiu, A. F., Whitehead, S. S., Adamus, J. E., Clements-Mann, M. L., Harris, D. O., Randolph, V. B., Udem, S. A., Murphy, B. R., et al. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.25.13961 · Cited by 420
Self-citationCollins, P. L., Hill, M. G., Cristina, J., & Grosfeld, H. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.1.81 · Cited by 296
Self-citationKarron, R. A., Wright, P. F., Belshe, R. B., Thumar, B., Casey, R., Newman, F., Polack, F. P., Randolph, V. B., Deatly, A., Hackell, J., et al. (2005) · The Journal of Infectious Diseases · doi:10.1086/427813 · Cited by 287
Self-citationLo, M. S., Brazas, R. M., & Holtzman, M. J. (2005) · Journal of Virology · doi:10.1128/jvi.79.14.9315-9319.2005 · Cited by 284
Metadata
- Authors
- Collins, P. L., Hill, M. G., Camargo, E., Grosfeld, H., Chanock, R. M., & Murphy, B. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1995-12-05
- Volume
- 92
- Issue
- 25
- Pages
- 11563-11567
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2068160897
- Cited by
- 450
- References
- 0
- FWCI
- 17.22
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Production of infectious human respiratory syncytial virus from cloned cDNA confirms an essential role for the transcription elongation factor from the 5' proximal open reading frame of the M2 mRNA in gene expression and provides a capability for vaccine development. https://app.cochat.ai/papers/10.1073/pnas.92.25.11563
- The paper
- Collins, P. L., Hill, M. G., Camargo, E., Grosfeld, H., Chanock, R. M., & Murphy, B. R. (1995). Production of infectious human respiratory syncytial virus from cloned cDNA confirms an essential role for the transcription elongation factor from the 5' proximal open reading frame of the M2 mRNA in gene expression and provides a capability for vaccine development. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.92.25.11563
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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