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Cited by 420

The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.

Respiratory syncytial virus (RSV) SH and G proteins are not essential for viral replication in vitro : Clinical evaluation and molecular characterization of a cold-passaged, attenuated RSV subgroup B mutant

Open access
Karron, Ruth A., Buonagurio, Deborah A., Georgiu, Alice F., Whitehead, Stephen S., Adamus, Jean E., Clements-Mann, Mary Lou, Harris, Denos O., Randolph, Valerie B., Udem, Stephen A., Murphy, Brian R., Sidhu, Mohinderjit S.·1997·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.94.25.13961
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Abstract

A live, cold-passaged ( cp ) candidate vaccine virus, designated respiratory syncytial virus (RSV) B1 cp -52/2B5 ( cp -52), replicated efficiently in Vero cells, but was found to be overattenuated for RSV-seronegative infants and children. Sequence analysis of reverse-transcription–PCR-amplified fragments of this mutant revealed a large deletion spanning most of the coding sequences for the small hydrophobic (SH) and attachment (G) proteins. Northern blot analysis of cp -52 detected multiple unique read-through mRNAs containing SH and G sequences, consistent with a deletion mutation spanning the SH:G gene junction. Immunological studies confirmed that an intact G glycoprotein was not produced by the cp -52 virus. Nonetheless, cp -52 was infectious and replicated to high titer in tissue culture despite the absence of the viral surface SH and G glycoproteins. Thus, our characterization of this negative-strand RNA virus identified a novel replication-competent deletion mutant lacking two of its three surface glycoproteins. The requirement of SH and G for efficient replication in vivo suggests that selective deletion of one or both of these RSV genes may provide an alternative or additive strategy for developing an optimally attenuated vaccine candidate.

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Metadata

Authors
Karron, R. A., Buonagurio, D. A., Georgiu, A. F., Whitehead, S. S., Adamus, J. E., Clements-Mann, M. L., Harris, D. O., Randolph, V. B., Udem, S. A., Murphy, B. R., & Sidhu, M. S.
Venue
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Published
1997-12-09
Volume
94
Issue
25
Pages
13961-13966
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.94.25.13961
OpenAlex
W2159482780
Cited by
420
References
21
FWCI
13.18
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Respiratory syncytial virus (RSV) SH and G proteins are not essential for viral replication in vitro : Clinical evaluation and molecular characterization of a cold-passaged, attenuated RSV subgroup B mutant. https://app.cochat.ai/papers/10.1073/pnas.94.25.13961
The paper
Karron, R. A., Buonagurio, D. A., Georgiu, A. F., Whitehead, S. S., Adamus, J. E., Clements-Mann, M. L., Harris, D. O., Randolph, V. B., Udem, S. A., Murphy, B. R., & Sidhu, M. S. (1997). Respiratory syncytial virus (RSV) SH and G proteins are not essential for viral replication in vitro : Clinical evaluation and molecular characterization of a cold-passaged, attenuated RSV subgroup B mutant. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.94.25.13961

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.