The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Polyadenylylation helps regulate mRNA decay in Escherichia coli.
Open accessAbstract
As part of our genetic analysis of mRNA decay in Escherichia coli K-12, we examined the effect of the pcnB gene [encoding poly(A) polymerase I] on message stability. Eliminating poly(A) polymerase I (delta pcnB) dramatically stabilized the lpp, ompA, and trxA transcripts. The half-lives of individual mRNAs were increased in both a delta pcnB single mutant and a delta pcnB pnp-7 rnb-500 rne-1 multiple mutant. We also found mRNA decay intermediates in delta pcnB mutants that were not detected in control strains. By end-labeling total E. coli RNA with [32P]pCp and T4 RNA ligase and then digesting the RNA with RNase A and T1, we showed that many RNAs in a wild-type strain contained poly(A) tails ranging from 10 nt to > 50 nt long. When polynucleotide phosphorylase, RNase II, and RNase E were absent, the length (> 100 nt) and number (10- to 20-fold) of the poly(A) tails increased. After transcription initiation was stopped with rifampicin, polyadenylylation apparently continued. Deleting the structural gene for poly(A) polymerase I (pcnB) reduced the amount of 3'-terminal poly(A) sequences by > 90%. We propose a model for the role of polyadenylylation in mRNA decay.
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Cites
Messenger RNA degradation in eukaryotes
Sachs, A. B. (1993) · Cell · doi:10.1016/0092-8674(93)80043-e · Cited by 846
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The critical role of RNA processing and degradation in the control of gene expression
Arraiano, C. M., Andrade, J. M., Domingues, S., Guinote, I. B., Malecki, M., Matos, R. G., Moreira, R. N., Pobre, V., Reis, F. P., Saramago, M., et al. (2010) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2010.00242.x · Cited by 349
Direct observation of processive exoribonuclease motion using optical tweezers
Fazal, F. M., Koslover, D. J., Luisi, B. F., & Block, S. M. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1514028112 · Cited by 29
Metadata
- Authors
- O'Hara, E. B., Chekanova, J. A., Ingle, C. A., Kushner, Z. R., Peters, E., & Kushner, S. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1995-03-14
- Volume
- 92
- Issue
- 6
- Pages
- 1807-1811
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2030230281
- Cited by
- 256
- References
- 1
- FWCI
- 4.97
- Type
- journal article
- Language
- en
- Open access
- green
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- O'Hara, E. B., Chekanova, J. A., Ingle, C. A., Kushner, Z. R., Peters, E., & Kushner, S. R. (1995). Polyadenylylation helps regulate mRNA decay in Escherichia coli. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.92.6.1807
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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