The DOI matches its Crossref record; 56 of the 56 references it cites were found in a registry or an index.
A New Yeast Poly(A) Polymerase Complex Involved in RNA Quality Control
Open accessAbstract
Eukaryotic cells contain several unconventional poly(A) polymerases in addition to the canonical enzymes responsible for the synthesis of poly(A) tails of nuclear messenger RNA precursors. The yeast protein Trf4p has been implicated in a quality control pathway that leads to the polyadenylation and subsequent exosome-mediated degradation of hypomethylated initiator tRNAMet (tRNAiMet). Here we show that Trf4p is the catalytic subunit of a new poly(A) polymerase complex that contains Air1p or Air2p as potential RNA-binding subunits, as well as the putative RNA helicase Mtr4p. Comparison of native tRNAiMet with its in vitro transcribed unmodified counterpart revealed that the unmodified RNA was preferentially polyadenylated by affinity-purified Trf4 complex from yeast, as well as by complexes reconstituted from recombinant components. These results and additional experiments with other tRNA substrates suggested that the Trf4 complex can discriminate between native tRNAs and molecules that are incorrectly folded. Moreover, the polyadenylation activity of the Trf4 complex stimulated the degradation of unmodified tRNAiMet by nuclear exosome fractions in vitro. Degradation was most efficient when coupled to the polyadenylation activity of the Trf4 complex, indicating that the poly(A) tails serve as signals for the recruitment of the exosome. This polyadenylation-mediated RNA surveillance resembles the role of polyadenylation in bacterial RNA turnover.
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RNA Quality Control in Eukaryotes
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Identification of a Nuclear Exosome Decay Pathway for Processed Transcripts
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Extensive Degradation of RNA Precursors by the Exosome in Wild-Type Cells
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Metadata
- Authors
- Vaňáčová, Š., Wolf, J., Martin, G., Blank, D., Dettwiler, S., Friedlein, A., Langen, H., Keith, G., & Keller, W.
- Venue
- PLoS Biology
- Publisher
- Public Library of Science (PLoS)
- Published
- 2005-04-19
- Volume
- 3
- Issue
- 6
- Pages
- e189
- ISSN
- 1544-9173 (ISSN-L)
- 1545-7885
- OpenAlex
- W2032501207
- Cited by
- 567
- References
- 56
- FWCI
- 17.63
- Type
- journal article
- Language
- en
- Open access
- gold
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- Vaňáčová, Š., Wolf, J., Martin, G., Blank, D., Dettwiler, S., Friedlein, A., Langen, H., Keith, G., & Keller, W. (2005). A New Yeast Poly(A) Polymerase Complex Involved in RNA Quality Control. PLoS Biology. https://doi.org/10.1371/journal.pbio.0030189
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