The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Efficiency of DNA replication in the polymerase chain reaction
Open accessAbstract
A detailed quantitative kinetic model for the polymerase chain reaction (PCR) is developed, which allows us to predict the probability of replication of a DNA molecule in terms of the physical parameters involved in the system. The important issue of the determination of the number of PCR cycles during which this probability can be considered to be a constant is solved within the framework of the model. New phenomena of multimodality and scaling behavior in the distribution of the number of molecules after a given number of PCR cycles are presented. The relevance of the model for quantitative PCR is discussed, and a novel quantitative PCR technique is proposed.
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“PCR is an exponential process, so small random variations early on in amplification can have a large impact on distribution18–24.”
From Quantifying Molecular Bias in DNA Data Storage (2019) · cites this paper as [19]
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Cited by
Sorted by citations
Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data
Ruijter, J. M., Ramakers, C., Hoogaars, W. M. H., Karlen, Y., Bakker, O., van den Hoff, M. J. B., & Moorman, A. F. M. (2009) · Nucleic Acids Research · doi:10.1093/nar/gkp045 · Cited by 3,043
Svec, D., Tichopad, A., Novosadova, V., Pfaffl, M. W., & Kubista, M. (2015) · Biomolecular Detection and Quantification · doi:10.1016/j.bdq.2015.01.005 · Cited by 601
Statistical significance of quantitative PCR
Karlen, Y., McNair, A., Perseguers, S., Mazza, C., & Mermod, N. (2007) · BMC Bioinformatics · doi:10.1186/1471-2105-8-131 · Cited by 373
Sources of PCR-induced distortions in high-throughput sequencing data sets
Kebschull, J. M., & Zador, A. M. (2015) · Nucleic Acids Research · doi:10.1093/nar/gkv717 · Cited by 300
Robust Quantification of Polymerase Chain Reactions Using Global Fitting
Carr, A. C., & Moore, S. D. (2012) · PLoS ONE · doi:10.1371/journal.pone.0037640 · Cited by 303
Metadata
- Authors
- Stolovitzky, G., & Cecchi, G.
- Affiliations
- Rockefeller University (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1996-11-12
- Volume
- 93
- Issue
- 23
- Pages
- 12947-12952
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC24026
- OpenAlex
- W2004803703
- Cited by
- 112
- References
- 23
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 5 · 2023: 4 · 2022: 3 · 2021: 8 · 2020: 8 · 2019: 1 · 2018: 2 · 2017: 2 · 2016: 7 · 2015: 5 · 2014: 5 · 2013: 4 · 2012: 5
- FWCI
- 0.50
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Physical Sciences › Mathematics › Mathematical Physics › Stochastic processes and statistical mechanics
- Keywords
- PCR, DNA replication, kinetic model, PCR cycle number, multimodality, scaling behavior, real-time PCR
- MeSH terms
- DNA Replication, Kinetics, Models, Theoretical, Probability, Time Factors, Reproducibility of Results, Polymerase Chain Reaction
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