The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Ligand-dependent development of the endothelial and hemopoietic lineages from embryonic mesodermal cells expressing vascular endothelial growth factor receptor 2
Open accessAbstract
The existence of a common precursor for endothelial and hemopoietic cells, termed the hemangioblast, has been postulated since the beginning of the century. Recently, deletion of the endothelial-specific vascular endothelial growth factor receptor 2 (VEGFR2) by gene targeting has shown that both endothelial and hemopoietic cells are absent in homozygous null mice. This observation suggested that VEGFR2 could be expressed by the hemangioblast and essential for its further differentiation along both lineages. However, it was not possible to exclude the hypothesis that hemopoietic failure was a secondary effect resulting from the absence of an endothelial cell microenvironment. To distinguish between these two hypotheses, we have produced a mAb directed against the extracellular domain of avian VEGFR2 and isolated VEGFR2+ cells from the mesoderm of chicken embryos at the gastrulation stage. We have found that in clonal cultures, a VEGFR2+ cell gives rise to either a hemopoietic or an endothelial cell colony. The developmental decision appears to be regulated by the binding of two different VEGFR2 ligands. Thus, endothelial differentiation requires VEGF, whereas hemopoietic differentiation occurs in the absence of VEGF and is significantly reduced by soluble VEGFR2, showing that this process could be mediated by a second, yet unidentified, VEGFR2 ligand. These observations thus suggest strongly that in the absence of the VEGFR2 gene product, the precursors of both hemopoietic and vascular endothelial lineages cannot survive. These cells therefore might be the initial targets of the VEGFR2 null mutation.
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Cites
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Cited by
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Mechanisms of normal and tumor-derived angiogenesis
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Cbfa2 is required for the formation of intra-aortic hematopoietic clusters
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Metadata
- Authors
- Eichmann, A., Corbel, C., Nataf, V., Vaigot, P., Bréant, C., & Le Douarin, N. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1997-05-13
- Volume
- 94
- Issue
- 10
- Pages
- 5141-5146
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2070081704
- Cited by
- 401
- References
- 26
- FWCI
- 8.32
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Ligand-dependent development of the endothelial and hemopoietic lineages from embryonic mesodermal cells expressing vascular endothelial growth factor receptor 2. https://app.cochat.ai/papers/10.1073/pnas.94.10.5141
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- Eichmann, A., Corbel, C., Nataf, V., Vaigot, P., Bréant, C., & Le Douarin, N. M. (1997). Ligand-dependent development of the endothelial and hemopoietic lineages from embryonic mesodermal cells expressing vascular endothelial growth factor receptor 2. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.94.10.5141
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
