The meso-angioblast: A multipotent, self-renewing cell that originates from the dorsal aorta and differentiates into most mesodermal tissues
Abstract
We have previously reported the origin of a class of skeletal myogenic cells from explants of dorsal aorta. This finding disagrees with the known origin of all skeletal muscle from somites and has therefore led us to investigate the in vivo origin of these cells and, moreover, whether their fate is restricted to skeletal muscle, as observed in vitro under the experimental conditions used. To address these issues, we grafted quail or mouse embryonic aorta into host chick embryos. Donor cells, initially incorporated into the host vessels, were later integrated into mesodermal tissues, including blood, cartilage, bone, smooth, skeletal and cardiac muscle. When expanded on a feeder layer of embryonic fibroblasts, the clonal progeny of a single cell from the mouse dorsal aorta acquired unlimited lifespan, expressed hemo-angioblastic markers (CD34, Flk1 and Kit) at both early and late passages, and maintained multipotency in culture or when transplanted into a chick embryo. We conclude that these newly identified vessel-associated stem cells, the meso-angioblasts, participate in postembryonic development of the mesoderm, and we speculate that postnatal mesodermal stem cells may be derived from a vascular developmental origin.
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Muscle regeneration involves non-resident progenitor cells with myogenic properties, such as mesoangioblasts, which upon injury are attracted to the site of damage where they can differentiate or fuse with pre-existing myofibers.
“Muscle regeneration is a very complex process involving sometimes non-resident progenitor cells with myogenic properties, such as mesoangioblasts. Upon injury, these cells are attracted to the site of damage where they can differentiate or fuse with pre-existing myofibers [72].”
From Adiponectin and Its Mimics on Skeletal Muscle: Insulin Sensitizers, Fat Burners, Exercise Mimickers, Muscling Pills … or Everything Together? (2020) · cites this paper as [72]
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- Authors
- Minasi, M. G., Riminucci, M., De Angelis, L., Borello, U., Berarducci, B., Innocenzi, A., Caprioli, A., Sirabella, D., Baiocchi, M., De Maria, R., Boratto, R., Jaffredo, T., Broccoli, V., Bianco, P., & Cossu, G.
- Affiliations
- Sapienza University of Rome (IT)
- University of L'Aquila (IT)
- Dipartimento di Istologia ed Embriologia Medica, Universita’ di Roma ‘La Sapienza’ Via Scarpa 14, 00161 Roma, Italy
- Stem Cell Research Institute, Dibit, H. S. Raffaele, Via Olgettina 58, 20132 Milano, Italy
- Centre National de la Recherche Scientifique (FR)
- Istituto Superiore di Sanità (IT)
- Laboratorio di Ematologia Oncologia, Istituto Superiore di Sanità, Viale Elena 297, 00161 Roma, Italy
- University of Pavia (IT)
- ORCID
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2002-06-01
- Volume
- 129
- Issue
- 11
- Pages
- 2773-2783
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W1938282147
- Cited by
- 514
- References
- 56
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 6 · 2023: 7 · 2022: 12 · 2021: 5 · 2020: 12 · 2019: 14 · 2018: 23 · 2017: 16 · 2016: 18 · 2015: 25 · 2014: 23 · 2013: 35 · 2012: 44
- FWCI
- 9.29
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Development (publishedVersion)
- PubMed (publishedVersion)
- CINECA IRIS Institutial research information system (University of Pisa) (submittedVersion)
- UCL Discovery (University College London) (submittedVersion)
- IRIS Research product catalog (Sapienza University of Rome) (submittedVersion)
- HighWire Press Open Archive (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Keywords
- dorsal aorta, mesoderm origin, multipotent stem cells, self-renewal, skeletal muscle, somites, cell fate, vascular development, angioblasts, CD34, Flk-1, KIT, cell transplantation, chick embryo
- MeSH terms
- Animals, Aorta, Cell Differentiation, Cells, Cultured, Coturnix, Mesoderm, Mice, Transgenic, Muscle, Smooth, Vascular, Cell Culture Techniques, Reverse Transcriptase Polymerase Chain Reaction, Mice
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