The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
A general purpose RNA-cleaving DNA enzyme
Open accessAbstract
An in vitro selection procedure was used to develop a DNA enzyme that can be made to cleave almost any targeted RNA substrate under simulated physiological conditions. The enzyme is comprised of a catalytic domain of 15 deoxynucleotides, flanked by two substrate-recognition domains of seven to eight deoxynucleotides each. The RNA substrate is bound through Watson–Crick base pairing and is cleaved at a particular phosphodiester located between an unpaired purine and a paired pyrimidine residue. Despite its small size, the DNA enzyme has a catalytic efficiency ( k cat / K m ) of ≈10 9 M −1 ⋅min −1 under multiple turnover conditions, exceeding that of any other known nucleic acid enzyme. Its activity is dependent on the presence of Mg 2+ ion. By changing the sequence of the substrate-recognition domains, the DNA enzyme can be made to target different RNA substrates. In this study, for example, it was directed to cleave synthetic RNAs corresponding to the start codon region of HIV-1 gag / pol , env , vpr , tat , and nef mRNAs.
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Cites
The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme
Guerrier-Takada, C., Gardiner, K., Marsh, T., Pace, N., & Altman, S. (1983) · Cell · doi:10.1016/0092-8674(83)90117-4 · Cited by 2,684
Thermodynamic parameters to predict stability of RNA/DNA hybrid duplexes
Sugimoto, N., Nakano, S.-I., Katoh, M., Matsumura, A., Nakamuta, H., Ohmichi, T., Yoneyama, M., & Sasaki, M. (1995) · Biochemistry · doi:10.1021/bi00035a029 · Cited by 697
Cited by
Dynamic DNA nanotechnology using strand-displacement reactions
Zhang, D. Y., & Seelig, G. (2011) · Nature Chemistry · doi:10.1038/nchem.957 · Cited by 1,785
Prebiotic Systems Chemistry: New Perspectives for the Origins of Life
Ruiz-Mirazo, K., Briones, C., & de la Escosura, A. (2014) · Chemical Reviews · doi:10.1021/cr2004844 · Cited by 930
Directed Evolution of Nucleic Acid Enzymes
Joyce, G. F. (2004) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.73.011303.073717 · Cited by 515
Self-citationEMILSSON, G. M., NAKAMURA, S., ROTH, A., & BREAKER, R. R. (2003) · RNA · doi:10.1261/rna.5680603 · Cited by 216
A ribozyme composed of only two different nucleotides
Reader, J. S., & Joyce, G. F. (2002) · Nature · doi:10.1038/nature01185 · Cited by 100
Self-citationWu, Y., Torabi, S.-F., Lake, R. J., Hong, S., Yu, Z., Wu, P., Yang, Z., Nelson, K., Guo, W., Pawel, G. T., et al. (2023) · Science Advances · doi:10.1126/sciadv.ade7622 · Cited by 91
Inhibition of miR-21 in glioma cells using catalytic nucleic acids
Belter, A., Rolle, K., Piwecka, M., Fedoruk-Wyszomirska, A., Naskręt-Barciszewska, M. Z., & Barciszewski, J. (2016) · Scientific Reports · doi:10.1038/srep24516 · Cited by 36
Metadata
- Authors
- Santoro, S. W., & Joyce, G. F.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1997-04-29
- Volume
- 94
- Issue
- 9
- Pages
- 4262-4266
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1977709085
- Cited by
- 1,541
- References
- 2
- FWCI
- 12.04
- Type
- journal article
- Language
- en
- Open access
- green
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