Simultaneous Fe 2+ /Fe 3+ imaging shows Fe 3+ over Fe 2+ enrichment in Alzheimer’s disease mouse brain
Open accessAbstract
Visualizing redox-active metal ions, such as Fe 2+ and Fe 3+ ions, are essential for understanding their roles in biological processes and human diseases. Despite the development of imaging probes and techniques, imaging both Fe 2+ and Fe 3+ simultaneously in living cells with high selectivity and sensitivity has not been reported. Here, we selected and developed DNAzyme-based fluorescent turn-on sensors that are selective for either Fe 2+ or Fe 3+ , revealing a decreased Fe 3+ /Fe 2+ ratio during ferroptosis and an increased Fe 3+ /Fe 2+ ratio in Alzheimer’s disease mouse brain. The elevated Fe 3+ /Fe 2+ ratio was mainly observed in amyloid plaque regions, suggesting a correlation between amyloid plaques and the accumulation of Fe 3+ and/or conversion of Fe 2+ to Fe 3+ . Our sensors can provide deep insights into the biological roles of labile iron redox cycling.
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The upregulation of TNF-α and IL-1β by TNE@AHAC is potentially attributed to TNE (Fe3O4-based nanozymes) liberated from the TNE@AHAC coating.
“an effect potentially attributed to TNE (Fe3O4-based nanozymes) liberated from the TNE@AHAC coating [61,62].”
From Nanocatalytic magnesium osteoimplants with biodegradable self-adaptive interfaces for therapeutic repair of infected bone defects (2026) · cites this paper as [62]
The Results section describes DNAzyme imaging of Fe²⁺ and Fe³⁺ in cells, and the Discussion addresses iron changes during ferroptosis; neither addresses TNE@AHAC. Searches for TNE@AHAC, Fe₃O₄ nanozymes, TNF-α, and IL-1β found no passage about coating release or cytokine upregulation. Page numbers were not available in our copy. (LLM, conf=0.76)
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- Authors
- Wu, Y., Torabi, S.-F., Lake, R. J., Hong, S., Yu, Z., Wu, P., Yang, Z., Nelson, K., Guo, W., Pawel, G. T., Van Stappen, J., Shao, X., Mirica, L. M., & Lu, Y.
- Affiliations
- University of Illinois Urbana-Champaign (US)
- Department of Chemistry, University of Texas at Austin, Austin, TX 78712, USA.
- Corresponding author
- Wu, Yuting
- Torabi, Seyed-Fakhreddin
- Lake, Ryan J.
- Lu, Yi
- ORCID
- Venue
- Science Advances
- Published
- 2023-04-21
- Volume
- 9
- Issue
- 16
- Pages
- eade7622-eade7622
- ISSN
- 2375-2548 (ISSN-L)
- PMC
- PMC10115418
- OpenAlex
- W4366383283
- Cited by
- 91
- References
- 92
- Citations by year
- 2026: 26 · 2025: 30 · 2024: 25 · 2023: 10
- FWCI
- 8.39
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Science Advances (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (R01GM114588, R01GM124316, R35GM141931)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Keywords
- iron redox cycling, fluorogenic probes, ferroptosis, Alzheimer's disease, amyloid plaques, labile iron, iron speciation, mouse brain
- MeSH terms
- Alzheimer Disease, Animals, Brain, Humans, Iron, Metals, Amyloid beta-Peptides, Mice, Plaque, Amyloid
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