Cited by 91Molecular Sensors and Ion DetectionAdvanced biosensing and bioanalysis techniquesAlzheimer's disease research and treatments

Simultaneous Fe 2+ /Fe 3+ imaging shows Fe 3+ over Fe 2+ enrichment in Alzheimer’s disease mouse brain

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Wu, Yuting, Torabi, Seyed-Fakhreddin, Lake, Ryan J., Hong, Shanni, Yu, Zhengxin, Wu, Peiwen, Yang, Zhenglin, Nelson, Kevin +6 more·2023·Science Advances

Abstract

Visualizing redox-active metal ions, such as Fe 2+ and Fe 3+ ions, are essential for understanding their roles in biological processes and human diseases. Despite the development of imaging probes and techniques, imaging both Fe 2+ and Fe 3+ simultaneously in living cells with high selectivity and sensitivity has not been reported. Here, we selected and developed DNAzyme-based fluorescent turn-on sensors that are selective for either Fe 2+ or Fe 3+ , revealing a decreased Fe 3+ /Fe 2+ ratio during ferroptosis and an increased Fe 3+ /Fe 2+ ratio in Alzheimer’s disease mouse brain. The elevated Fe 3+ /Fe 2+ ratio was mainly observed in amyloid plaque regions, suggesting a correlation between amyloid plaques and the accumulation of Fe 3+ and/or conversion of Fe 2+ to Fe 3+ . Our sensors can provide deep insights into the biological roles of labile iron redox cycling.

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Crossref · 10.1126/sciadv.ade7622

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  • The upregulation of TNF-α and IL-1β by TNE@AHAC is potentially attributed to TNE (Fe3O4-based nanozymes) liberated from the TNE@AHAC coating.

    “an effect potentially attributed to TNE (Fe3O4-based nanozymes) liberated from the TNE@AHAC coating [61,62].”

    From Nanocatalytic magnesium osteoimplants with biodegradable self-adaptive interfaces for therapeutic repair of infected bone defects (2026) · cites this paper as [62]

    The Results section describes DNAzyme imaging of Fe²⁺ and Fe³⁺ in cells, and the Discussion addresses iron changes during ferroptosis; neither addresses TNE@AHAC. Searches for TNE@AHAC, Fe₃O₄ nanozymes, TNF-α, and IL-1β found no passage about coating release or cytokine upregulation. Page numbers were not available in our copy. (LLM, conf=0.76)

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Authors
Wu, Y., Torabi, S.-F., Lake, R. J., Hong, S., Yu, Z., Wu, P., Yang, Z., Nelson, K., Guo, W., Pawel, G. T., Van Stappen, J., Shao, X., Mirica, L. M., & Lu, Y.
Affiliations
  • University of Illinois Urbana-Champaign (US)
  • Department of Chemistry, University of Texas at Austin, Austin, TX 78712, USA.
Corresponding author
  • Wu, Yuting
  • Torabi, Seyed-Fakhreddin
  • Lake, Ryan J.
  • Lu, Yi
Venue
Science Advances
Published
2023-04-21
Volume
9
Issue
16
Pages
eade7622-eade7622
ISSN
2375-2548 (ISSN-L)
OpenAlex
W4366383283
Cited by
91
References
92
Citations by year
2026: 26 · 2025: 30 · 2024: 25 · 2023: 10
FWCI
8.39
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Science Advances (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
National Institute of General Medical Sciences (R01GM114588, R01GM124316, R35GM141931)
Topics
  • Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
Keywords
iron redox cycling, fluorogenic probes, ferroptosis, Alzheimer's disease, amyloid plaques, labile iron, iron speciation, mouse brain
MeSH terms
Alzheimer Disease, Animals, Brain, Humans, Iron, Metals, Amyloid beta-Peptides, Mice, Plaque, Amyloid

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