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Visualization of elongation factor G on the Escherichia coli 70S ribosome: The mechanism of translocation
Open accessAbstract
During protein synthesis, elongation factor G (EF-G) binds to the ribosome and promotes the step of translocation, a process in which tRNA moves from the A to the P site of the ribosome and the mRNA is advanced by one codon. By using three-dimensional cryo-electron microscopy, we have visualized EF-G in a ribosome–EF-G–GDP–fusidic acid complex. Fitting the crystal structure of EF-G–GDP into the cryo density map reveals a large conformational change mainly associated with domain IV, the domain that mimics the shape of the anticodon arm of the tRNA in the structurally homologous ternary complex of Phe-tRNA Phe , EF-Tu, and a GTP analog. The tip portion of this domain is found in a position that overlaps the anticodon arm of the A-site tRNA, whose position in the ribosome is known from a study of the pretranslocational complex, implying that EF-G displaces the A-site tRNA to the P site by physical interaction with the anticodon arm.
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Cited by
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Self-citationThe Structure of the Ribosome with Elongation Factor G Trapped in the Posttranslocational State
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Taylor, D. J., Nilsson, J., Merrill, A. R., Andersen, G. R., Nissen, P., & Frank, J. (2007) · The EMBO Journal · doi:10.1038/sj.emboj.7601677 · Cited by 193
Self-citationRole of Domains 4 and 5 in Elongation Factor G Functions on the Ribosome
Savelsbergh, A., Matassova, N. B., Rodnina, M. V., & Wintermeyer, W. (2000) · Journal of Molecular Biology · doi:10.1006/jmbi.2000.3886 · Cited by 115
Cameron, D. M., Thompson, J., March, P. E., & Dahlberg, A. E. (2002) · Journal of Molecular Biology · doi:10.1016/s0022-2836(02)00235-8 · Cited by 99
Spahn, C. M. T., Blaha, G., Agrawal, R. K., Penczek, P., Grassucci, R. A., Trieber, C. A., Connell, S. R., Taylor, D. E., Nierhaus, K. H., & Frank, J. (2001) · Molecular Cell · doi:10.1016/s1097-2765(01)00238-6 · Cited by 81
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Self-citationDomain IV of elongation factor G from Thermus thermophilus is strictly required for translocation
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Metadata
- Authors
- Agrawal, R. K., Penczek, P., Grassucci, R. A., & Frank, J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1998-05-26
- Volume
- 95
- Issue
- 11
- Pages
- 6134-6138
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2152348402
- Cited by
- 325
- References
- 0
- FWCI
- 11.59
- Type
- journal article
- Language
- en
- Open access
- green
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