The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Systematic identification of essential genes by in vitro mariner mutagenesis
Open accessAbstract
Although the complete DNA sequences of several microbial genomes are now available, nearly 40% of the putative genes lack identifiable functions. Comprehensive screens and selections for identifying functional classes of genes are needed to convert sequence data into meaningful biological information. One particularly significant group of bacterial genes consists of those that are essential for growth or viability. Here, we describe a simple system for performing transposon mutagenesis on naturally transformable organisms along with a technique to rapidly identify essential or conditionally essential DNA segments. We show the general utility of this approach by applying it to two human pathogens, Haemophilus influenzae and Streptococcus pneumoniae , in which we detected known essential genes and assigned essentiality to several ORFs of unknown function.
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Citations
Cites
The complete genome sequence of the gastric pathogen Helicobacter pylori
Tomb, J.-F., White, O., Kerlavage, A. R., Clayton, R. A., Sutton, G. G., Fleischmann, R. D., Ketchum, K. A., Klenk, H. P., Gill, S., Dougherty, B. A., et al. (1997) · Nature · doi:10.1038/41483 · Cited by 3,529
A minimal gene set for cellular life derived by comparison of complete bacterial genomes
Mushegian, A. R., & Koonin, E. V. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.19.10268 · Cited by 916
Cited by
Griffin, J. E., Gawronski, J. D., DeJesus, M. A., Ioerger, T. R., Akerley, B. J., & Sassetti, C. M. (2011) · PLoS Pathogens · doi:10.1371/journal.ppat.1002251 · Cited by 1,048
Self-citationvan Opijnen, T., Bodi, K. L., & Camilli, A. (2009) · Nature Methods · doi:10.1038/nmeth.1377 · Cited by 933
Self-citationAdvances in In Vivo Bioluminescence Imaging of Gene Expression
Contag, C. H., & Bachmann, M. H. (2002) · Annual Review of Biomedical Engineering · doi:10.1146/annurev.bioeng.4.111901.093336 · Cited by 870
Comprehensive identification of conditionally essential genes in mycobacteria
Sassetti, C. M., Boyd, D. H., & Rubin, E. J. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.231275498 · Cited by 616
Self-citationTransposon insertion sequencing: a new tool for systems-level analysis of microorganisms
van Opijnen, T., & Camilli, A. (2013) · Nature Reviews Microbiology · doi:10.1038/nrmicro3033 · Cited by 494
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Metadata
- Authors
- Akerley, B. J., Rubin, E. J., Camilli, A., Lampe, D. J., Robertson, H. M., & Mekalanos, J. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1998-07-21
- Volume
- 95
- Issue
- 15
- Pages
- 8927-8932
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2013902051
- Cited by
- 271
- References
- 2
- FWCI
- 10.43
- Type
- journal article
- Language
- en
- Open access
- green
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- Akerley, B. J., Rubin, E. J., Camilli, A., Lampe, D. J., Robertson, H. M., & Mekalanos, J. J. (1998). Systematic identification of essential genes by in vitro mariner mutagenesis. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.95.15.8927
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