The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
A previously unidentified host protein protects retroviral DNA from autointegration
Open accessAbstract
Integration of a DNA copy of the viral genome into a host chromosome is an essential step in the retrovirus life cycle. The machinery that carries out the integration reaction is a nucleoprotein complex derived from the core of the infecting virion. To successfully integrate into host DNA, the viral DNA within this complex must avoid self-destructive integration into itself, a reaction termed autointegration. We have previously shown [Lee, M. S. and Craigie, R. (1994) Proc. Natl. Acad. Sci. USA 91, 9823–9827] that viral nucleoprotein complexes isolated from Moloney murine leukemia virus-infected cells exhibit a barrier to autointegration. This autointegration barrier could be destroyed by stripping factors from the complexes and subsequently restored by incubation with a host cell extract, but not by incubation with an extract of disrupted virions. We have now used this autointegration barrier reconstitution assay to purify the host factor from uninfected NIH 3T3 fibroblasts. It is a single polypeptide of 89 aa that does not match any previously identified protein. The identity of the protein was confirmed by expressing it in Escherichia coli and demonstrating the activity of the heterologously expressed protein in the reconstitution assay.
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Cites
Bushman, F. D., & Craigie, R. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.4.1339 · Cited by 410
Self-citationBrown, P. O., Bowerman, B., Varmus, H. E., & Bishop, J. M. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.8.2525 · Cited by 399
Retroviral DNA integration: structure of an integration intermediate
Fujiwara, T., & Mizuuchi, K. (1988) · Cell · doi:10.1016/0092-8674(88)90071-2 · Cited by 385
Retroviral DNA integration directed by HIV integration protein in vitro
Bushman, F. D., Fujiwara, T., & Craigie, R. (1990) · Science · doi:10.1126/science.2171144 · Cited by 338
Self-citationKatzman, M., Katz, R. A., Skalka, A. M., & Leis, J. (1989) · Journal of Virology · doi:10.1128/jvi.63.12.5319-5327.1989 · Cited by 311
Schwartzberg, P., Colicelli, J., & Goff, S. P. (1984) · Cell · doi:10.1016/0092-8674(84)90439-2 · Cited by 242
Relationship of avian retrovirus DNA synthesis to integration in vitro.
Lee, Y. M., & Coffin, J. M. (1991) · Molecular and Cellular Biology · doi:10.1128/mcb.11.3.1419 · Cited by 77
Cited by
HIV-1 Integrase Forms Stable Tetramers and Associates with LEDGF/p75 Protein in Human Cells
Cherepanov, P., Maertens, G., Proost, P., Devreese, B., Van Beeumen, J., Engelborghs, Y., De Clercq, E., & Debyser, Z. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m209278200 · Cited by 704
Nuclear lamins: building blocks of nuclear architecture
Goldman, R. D., Gruenbaum, Y., Moir, R. D., Shumaker, D. K., & Spann, T. P. (2002) · Genes & Development · doi:10.1101/gad.960502 · Cited by 586
Mechanisms generating cancer genome complexity from a single cell division error
Umbreit, N. T., Zhang, C.-Z., Lynch, L. D., Blaine, L. J., Cheng, A. M., Tourdot, R., Sun, L., Almubarak, H. F., Judge, K., Mitchell, T. J., et al. (2020) · Science · doi:10.1126/science.aba0712 · Cited by 439
Craigie, R., & Bushman, F. D. (2012) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a006890 · Cited by 327
Self-citationCapsid Is a Dominant Determinant of Retrovirus Infectivity in Nondividing Cells
Yamashita, M., & Emerman, M. (2004) · Journal of Virology · doi:10.1128/jvi.78.11.5670-5678.2004 · Cited by 325
Metadata
- Authors
- Lee, M. S., & Craigie, R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1998-02-17
- Volume
- 95
- Issue
- 4
- Pages
- 1528-1533
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1973779048
- Cited by
- 281
- References
- 7
- FWCI
- 4.22
- Type
- journal article
- Language
- en
- Open access
- green
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
