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Agrobacterium-transformed rice plants expressing synthetic cryIA(b)and cryIA(c)genes are highly toxic to striped stem borer and yellow stem borer
Open accessAbstract
Over 2,600 transgenic rice plants in nine strains were regenerated from >500 independently selected hygromycin-resistant calli after Agrobacterium-mediated transformation. The plants were transformed with fully modified (plant codon optimized) versions of two synthetic cryIA(b)and cryIA(c)coding sequences from Bacillus thuringiensis as well as the hph and gus genes, coding for hygromycin phosphotransferase and β-glucuronidase, respectively. These sequences were placed under control of the maize ubiquitin promoter, the CaMV35S promoter, and the Brassica Bp10 gene promoter to achieve high and tissue-specific expression of the lepidopteran-specific δ-endotoxins. The integration, expression, and inheritance of these genes were demonstrated in R 0 and R 1 generations by Southern, Northern, and Western analyses and by other techniques. Accumulation of high levels (up to 3% of soluble proteins) of CryIA(b) and CryIA(c) proteins was detected in R 0 plants. Bioassays with R 1 transgenic plants indicated that the transgenic plants were highly toxic to two major rice insect pests, striped stem borer (Chilo suppressalis) and yellow stem borer (Scirpophaga incertulas), with mortalities of 97–100% within 5 days after infestation, thus offering a potential for effective insect resistance in transgenic rice plants.
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Cited by
Bernal, C. C., Aguda, R. M., & Cohen, M. B. (2002) · Entomologia Experimentalis et Applicata · doi:10.1046/j.1570-7458.2002.00921.x · Cited by 108
Breitler, J. C., Vassal, J. M., Del Mar Catala, M., Meynard, D., Marfà, V., Melé, E., Royer, M., Murillo, I., San Segundo, B., Guiderdoni, E., et al. (2004) · Plant Biotechnology Journal · doi:10.1111/j.1467-7652.2004.00086.x · Cited by 93
Breitler, J. C., Cordero, M. J., Royer, M., Meynard, D., San Segundo, B., & Guiderdoni, E. (2001) · Molecular Breeding · doi:10.1023/a:1011609128319 · Cited by 48
Anayol, E., Bakhsh, A., Karakoç, Ö. C., Onarıcı, S., Köm, D., Aasim, M., Özcan, S. F., Barpete, S., Khabbazi, S. D., Önol, B., et al. (2016) · Plant Biotechnology Reports · doi:10.1007/s11816-016-0388-5 · Cited by 16
Metadata
- Authors
- Cheng, X., Sardana, R., Kaplan, H., & Altosaar, I.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1998-03-17
- Volume
- 95
- Issue
- 6
- Pages
- 2767-2772
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1980020410
- Cited by
- 354
- References
- 0
- FWCI
- 6.64
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Agrobacterium-transformed rice plants expressing synthetic cryIA(b)and cryIA(c)genes are highly toxic to striped stem borer and yellow stem borer. https://app.cochat.ai/papers/10.1073/pnas.95.6.2767
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- Cheng, X., Sardana, R., Kaplan, H., & Altosaar, I. (1998). Agrobacterium-transformed rice plants expressing synthetic cryIA(b)and cryIA(c)genes are highly toxic to striped stem borer and yellow stem borer. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.95.6.2767
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