The DOI matches its Crossref record; 27 of the 29 references it cites were found in a registry or an index, and 2 are not checked yet.
Homology-directed repair is a major double-strand break repair pathway in mammalian cells
Open accessAbstract
Mammalian cells have been presumed to repair potentially lethal chromosomal double-strand breaks (DSBs) in large part by processes that do not require homology to the break site. This contrasts with Saccharomyces cerevisiae where the major DSB repair pathway is homologous recombination. Recently, it has been determined that DSBs in genomic DNA in mammalian cells can stimulate homologous recombination as much as 3 or 4 orders of magnitude, suggesting that homology-directed repair may play an important role in the repair of chromosomal breaks. To determine whether mammalian cells use recombinational repair at a significant level, we have analyzed the spectrum of repair events at a defined chromosomal break by using direct physical analysis of repair products. When an endonuclease-generated DSB is introduced into one of two direct repeats, homologous repair is found to account for 30–50% of observed repair events. Both noncrossover and deletional homologous repair products are detected, at approximately a 1:3 ratio. These results demonstrate the importance of homologous recombination in the repair of DSBs in mammalian cells. In the remaining observed repair events, DSBs are repaired by nonhomologous processes. The nonhomologous repair events generally result in small deletions or insertions at the break site, although a small fraction of events result in larger chromosomal rearrangements. Interestingly, in two insertions, GT repeats were integrated at one of the broken chromosome ends, suggesting that DSB repair can contribute to the spread of microsatellite sequences in mammalian genomes.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Alternatively, the HDR pathway is capable of repairing DSBs using a sister chromatid as a repair template, and for this reason, it is considered an error‐free system.”
From CRISPR/Cas9 Ribonucleoprotein Nucleofection for Genome Editing in Primary Human Keratinocytes: Knockouts, Deletions, and Homology‐Directed Repair Mutagenesis (2024) · cites this paper as Liang et al., 1998
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Molecular Cloning. A Laboratory Manual
Wood, E. (1983) · Biochemical Education · doi:10.1016/0307-4412(83)90068-7 · Cited by 29,534
The double-strand-break repair model for recombination
Szostak, J. W., Orr-Weaver, T. L., Rothstein, R. J., & Stahl, F. W. (1983) · Cell · doi:10.1016/0092-8674(83)90331-8 · Cited by 2,437
Hamada, H., Petrino, M. G., & Kakunaga, T. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.21.6465 · Cited by 743
Rouet, P., Smih, F., & Jasin, M. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.12.8096 · Cited by 739
Self-citationExpression of a site-specific endonuclease stimulates homologous recombination in mammalian cells.
Rouet, P., Smih, F., & Jasin, M. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.13.6064 · Cited by 571
Self-citationChoulika, A., Perrin, A., Dujon, B., & Nicolas, J.-F. (1995) · Molecular and Cellular Biology · doi:10.1128/mcb.15.4.1968 · Cited by 459
Genetic manipulation of genomes with rare-cutting endonucleases
Jasin, M. (1996) · Trends in Genetics · doi:10.1016/0168-9525(96)10019-6 · Cited by 391
Self-citationEvolution and distribution of (GT)n repetitive sequences in mammalian genomes
Stallings, R. L., Ford, A., Nelson, D., Torney, D. C., Hildebrand, C. E., & Moyzis, R. K. (1991) · Genomics · doi:10.1016/0888-7543(91)90467-s · Cited by 379
Rouet, P., Smih, F., & Jasin, M. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.12.8096-8106.1994 · Cited by 363
Self-citationDNA double-strand break repair and V(D)J recombination: involvement of DNA-PK
Jackson, S. P., & Jeggo, P. A. (1995) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(00)89090-8 · Cited by 345
LOSS OF CONSTITUTIONAL HETEROZYGOSITY IN HUMAN CANCER
Lasko, D. D., Cavenee, W. K., & Nordenskjöld, M. (1991) · Annual Review of Genetics · doi:10.1146/annurev.ge.25.120191.001433 · Cited by 314
Gene Conversion Tracts from Double-Strand Break Repair in Mammalian Cells
Elliott, B., Richardson, C. A., Winderbaum, J., Nickoloff, J. A., & Jasin, M. (1998) · Molecular and Cellular Biology · doi:10.1128/mcb.18.1.93 · Cited by 305
Self-citationThe sequence (dC-dA)n X (dG-dT)n forms left-handed Z-DNA in negatively supercoiled plasmids.
Nordheim, A., & Rich, A. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.7.1821 · Cited by 302
Sargent, R. G., Brenneman, M. A., & Wilson, J. H. (1997) · Molecular and Cellular Biology · doi:10.1128/mcb.17.1.267 · Cited by 271
Homologous recombination and the roles of double-strand breaks
Shinohara, A., & Ogawa, T. (1995) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(00)89085-4 · Cited by 236
Double-strand breaks at the target locus stimulate gene targeting in embryonic stem cells
Smih, F., Rouet, P., Romanienko, P. J., & Jasin, M. (1995) · Nucleic Acids Research · doi:10.1093/nar/23.24.5012 · Cited by 220
Self-citationChromosomal double-strand break repair in Ku80-deficient cells.
Liang, F., Romanienko, P. J., Weaver, D. T., Jeggo, P. A., & Jasin, M. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.17.8929 · Cited by 190
Self-citationIn vivo biochemistry: Physical monitoring of recombination induced by site‐specific endonucleases
Haber, J. E. (1995) · BioEssays · doi:10.1002/bies.950170707 · Cited by 188
Loss of heterozygosity induced by a chromosomal double-strand break
Moynahan, M. E., & Jasin, M. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.17.8988 · Cited by 186
Self-citationChambers, S. R., Hunter, N., Louis, E. J., & Borts, R. H. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.11.6110 · Cited by 181
Chromosomal Double-Strand Breaks Induce Gene Conversion at High Frequency in Mammalian Cells
Taghian, D. G., & Nickoloff, J. A. (1997) · Molecular and Cellular Biology · doi:10.1128/mcb.17.11.6386 · Cited by 172
Schichman, S. A., Caligiuri, M. A., Strout, M. P., Carter, S. L., Gu, Y., Canaani, E., Bloomfield, C. D., & Croce, C. M. (1994) · PubMed · Cited by 165
Homologous integration in mammalian cells without target gene selection.Preprint
Jasin, M., & Berg, P. R. (1988) · Genes & Development · doi:10.1101/gad.2.11.1353 · Cited by 165
Self-citationIllegitimate recombination induced by DNA double-strand breaks in a mammalian chromosome.
Phillips, J. W., & Morgan, W. F. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.9.5794 · Cited by 117
Lin, F., Sperle, K., & Sternberg, N. L. (1990) · Molecular and Cellular Biology · doi:10.1128/mcb.10.1.103 · Cited by 112
Lukácsovich, T., Yang, D., & Waldman, A. S. (1994) · Nucleic Acids Research · doi:10.1093/nar/22.25.5649 · Cited by 99
Meiotic Recombination in Yeast: Coronation of the Double-Strand-Break Repair Model
Stahl, F. (1996) · Cell · doi:10.1016/s0092-8674(00)81791-2 · Cited by 93
Jasin, M., de Villiers, J., Weber, F., & Schaffner, W. (1985) · Cell · doi:10.1016/0092-8674(85)90242-9 · Cited by 90
Self-citationIllegitimate Recombination Induced by DNA Double-Strand Breaks in a Mammalian Chromosome
Phillips, J. W., & Morgan, W. F. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.9.5794-5803.1994 · Cited by 49
Flagged first, then by citations
Research works printed but not linked: 3
- T D Petes, R E Malone, L S Symington Recombination in Yeast (Cold Spring Harbor Lab. Press, Plainview, NY, 1991).
- 10.1128/mcb.10.1.103-112.1990 · DOI
- S A Schichman, M A Caligiuri, M P Strout, S L Carter, Y Gu, E Canaani, C D Bloomfield, C M Croce Cancer Res 54, 4277–4280 (1994).
Cited by
Sorted by citations
XRCC3 promotes homology-directed repair of DNA damage in mammalian cellsPreprint
Pierce, A. J., Johnson, R. D., Thompson, L. H., & Jasin, M. (1999) · Genes & Development · doi:10.1101/gad.13.20.2633 · Cited by 1,384
Self-citationBrca1 Controls Homology-Directed DNA Repair
Moynahan, M. E., Chiu, J. W., Koller, B. H., & Jasin, M. (1999) · Molecular Cell · doi:10.1016/s1097-2765(00)80202-6 · Cited by 1,245
Self-citationBRCA2 Is Required for Homology-Directed Repair of Chromosomal Breaks
Moynahan, M. E., Pierce, A. J., & Jasin, M. (2001) · Molecular Cell · doi:10.1016/s1097-2765(01)00174-5 · Cited by 1,007
Self-citationAn improved zinc-finger nuclease architecture for highly specific genome editing
Miller, J. C., Holmes, M. C., Wang, J., Guschin, D. Y., Lee, Y.-L., Rupniewski, I., Beausejour, C. M., Waite, A. J., Wang, N. S., Kim, K. A., et al. (2007) · Nature Biotechnology · doi:10.1038/nbt1319 · Cited by 1,051
Molecular views of recombination proteins and their control
West, S. C. (2003) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1127 · Cited by 977
Metadata
- Authors
- Liang, F., Han, M., Romanienko, P. J., & Jasin, M.
- Affiliations
- Cornell University (US)
- Corresponding author
- Jasin, Maria
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1998-04-28
- Volume
- 95
- Issue
- 9
- Pages
- 5172-5177
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC20233
- OpenAlex
- W2168809955
- Cited by
- 649
- References
- 27
- Citations by year
- 2026: 5 · 2025: 11 · 2024: 18 · 2023: 12 · 2022: 21 · 2021: 25 · 2020: 19 · 2019: 17 · 2018: 13 · 2017: 16 · 2016: 13 · 2015: 13 · 2014: 12 · 2013: 11 · 2012: 13
- FWCI
- 15.73
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- American Cancer Society
- National Institutes of Health
- National Institute of General Medical Sciences (GM54688)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
- Keywords
- homology-directed repair, DNA double-strand break repair, homologous recombination, non-homologous end joining, mammalian cells, chromosomal breaks, chromosomal rearrangements
- MeSH terms
- Animals, Cells, Cultured, DNA Damage, DNA Repair, Gene Conversion, Cricetinae, Recombination, Genetic, Sequence Homology, Nucleic Acid, Restriction Mapping, CHO Cells, Sequence Deletion
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
