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Patterning cells and their environments using multiple laminar fluid flows in capillary networks
Open accessAbstract
This paper describes the use of laminar flow of liquids in capillary systems to pattern the cell culture substrate, to perform patterned cell deposition, and to pattern the cell culture media. We demonstrate the patterning of the cell culture substrate with different proteins, the patterning of different types of cells adjacent to each other, the patterned delivery of chemicals to adhered cells, and performing enzymatic reactions over select cells or over a portion of a cell. This method offers a way to simultaneously control the characteristics of the surface to which cells are attached, the type of cells that are in their vicinity, and the kind of media that cells or part of a cell are exposed to. The method is experimentally simple, highly adaptable, and requires no special equipment except for an elastomeric relief that can be readily prepared by rapid prototyping.
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Citations
Cited by
Microfluidic cell culture systems for drug research
Wu, M.-H., Huang, S.-B., & Lee, G.-B. (2010) · Lab on a Chip · doi:10.1039/b921695b · Cited by 432
Biological applications of microfluidic gradient devices
Kim, S., Kim, H. J., & Jeon, N. L. (2010) · Integrative Biology · doi:10.1039/c0ib00055h · Cited by 366
Engineering cellular microenvironments to improve cell-based drug testing
Bhadriraju, K., & Chen, C. S. (2002) · Drug Discovery Today · doi:10.1016/s1359-6446(02)02273-0 · Cited by 355
Tokeshi, M., Minagawa, T., Uchiyama, K., Hibara, A., Sato, K., Hisamoto, H., & Kitamori, T. (2002) · Analytical Chemistry · doi:10.1021/ac011111z · Cited by 342
Layer-by-layer microfluidics for biomimetic three-dimensional structures
Tan, W., & Desai, T. A. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2003.08.021 · Cited by 193
Metadata
- Authors
- Takayama, S., McDonald, J. C., Ostuni, E., Liang, M. N., Kenis, P. J. A., Ismagilov, R. F., & Whitesides, G. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1999-05-11
- Volume
- 96
- Issue
- 10
- Pages
- 5545-5548
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2043938126
- Cited by
- 528
- References
- 0
- FWCI
- 20.32
- Type
- journal article
- Language
- en
- Open access
- green
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- The paper
- Takayama, S., McDonald, J. C., Ostuni, E., Liang, M. N., Kenis, P. J. A., Ismagilov, R. F., & Whitesides, G. M. (1999). Patterning cells and their environments using multiple laminar fluid flows in capillary networks. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.96.10.5545
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
