The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Thermodynamic basis of the enhanced specificity of structured DNA probes
Open accessAbstract
Molecular beacons are DNA probes that form a stem-and-loop structure and possess an internally quenched fluorophore. When they bind to complementary nucleic acids, they undergo a conformational transition that switches on their fluorescence. These probes recognize their targets with higher specificity than probes that cannot form a hairpin stem, and they easily discriminate targets that differ from one another by only a single nucleotide. Our results show that molecular beacons can exist in three different states: bound to a target, free in the form of a hairpin structure, and free in the form of a random coil. Thermodynamic analysis of the transitions between these states reveals that enhanced specificity is a general feature of conformationally constrained probes.
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Cites
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Metadata
- Authors
- Bonnet, G., Tyagi, S., Libchaber, A., & Kramer, F. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1999-05-25
- Volume
- 96
- Issue
- 11
- Pages
- 6171-6176
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1996491588
- Cited by
- 658
- References
- 10
- FWCI
- 7.18
- Type
- journal article
- Language
- en
- Open access
- green
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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