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Covalent modification of the homeodomain-interacting protein kinase 2 (HIPK2) by the ubiquitin-like protein SUMO-1
Open accessAbstract
Posttranslational modifications such as ubiquitination and phosphorylation play an important role in the regulation of cellular protein function. Homeodomain-interacting protein kinase 2 (HIPK2) is a member of the recently identified family of nuclear protein kinases that act as corepressors for homeodomain transcription factors. Here, we show that HIPK2 is regulated by a ubiquitin-like protein, SUMO-1. We demonstrate that HIPK2 localizes to nuclear speckles (dots) by means of a speckle-retention signal. This speckle-retention signal contains a domain that interacts with a mouse ubiquitin-like protein conjugating (E2) enzyme, mUBC9. In cultured cells, HIPK2 is covalently modified by SUMO-1, and the SUMO-1 modification of HIPK2 correlates with its localization to nuclear speckles (dots). Thus, our results provide firm evidence that the nuclear protein kinase HIPK2 can be covalently modified by SUMO-1, which directs its localization to nuclear speckles (dots).
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Cited by
Hunter, T. (2000) · Cell · doi:10.1016/s0092-8674(00)81688-8 · Cited by 2,586
Post-translational modification of p53 in tumorigenesis
Bode, A. M., & Dong, Z. (2004) · Nature Reviews Cancer · doi:10.1038/nrc1455 · Cited by 1,270
Kang, R., Chen, R., Zhang, Q., Hou, W., Wu, S., Cao, L., Huang, J., Yu, Y., Fan, X.-G., Yan, Z., et al. (2014) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2014.05.001 · Cited by 956
Comerford, K. M., Leonard, M. O., Karhausen, J., Carey, R., Colgan, S. P., & Taylor, C. T. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0337412100 · Cited by 185
Chen, W.-Y., Lee, W.-C., Hsu, N.-C., Huang, F., & Chung, B.-C. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m405006200 · Cited by 104
Metadata
- Authors
- Kim, Y. H., Choi, C. Y., & Kim, Y.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1999-10-26
- Volume
- 96
- Issue
- 22
- Pages
- 12350-12355
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2072339454
- Cited by
- 161
- References
- 0
- FWCI
- 5.65
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Covalent modification of the homeodomain-interacting protein kinase 2 (HIPK2) by the ubiquitin-like protein SUMO-1. https://app.cochat.ai/papers/10.1073/pnas.96.22.12350
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- Kim, Y. H., Choi, C. Y., & Kim, Y. (1999). Covalent modification of the homeodomain-interacting protein kinase 2 (HIPK2) by the ubiquitin-like protein SUMO-1. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.96.22.12350
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