The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Cyclin-dependent kinase control of centrosome duplication
Open accessAbstract
Centrosomes nucleate microtubules and duplicate once per cell cycle. This duplication and subsequent segregation in mitosis results in maintenance of the one centrosome/cell ratio. Centrosome duplication occurs during the G 1 /S transition in somatic cells and must be coupled to the events of the nuclear cell cycle; failure to coordinate duplication and mitosis results in abnormal numbers of centrosomes and aberrant mitoses. Using both in vivo and in vitro assays, we show that centrosome duplication in Xenopus laevis embryos requires cyclin/cdk2 kinase activity. Injection of the cdk (cyclin-dependent kinase) inhibitor p21 into one blastomere of a dividing embryo blocks centrosome duplication in that blastomere; the related cdk inhibitor p27 has a similar effect. An in vitro system using Xenopus extracts carries out separation of the paired centrioles within the centrosome. This centriole separation activity is dependent on cyclin/cdk2 activity; depletion of either cdk2 or of the two activating cyclins, cyclin A and cyclin E, eliminates centriole separation activity. In addition, centriole separation is inhibited by the mitotic state, suggesting a mechanism of linking the cell cycle to periodic duplication of the centrosome.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Separate domains of p21 involved in the inhibition of Cdk kinase and PCNA
Chen, J., Jackson, P. K., Kirschner, M. W., & Dutta, A. (1995) · Nature · doi:10.1038/374386a0 · Cited by 548
Self-citation
Cited by
Centrosome duplication in mammalian somatic cells requires E2F and Cdk2–Cyclin A
Meraldi, P., Lukas, J., Fry, A. M., Bartek, J., & Nigg, E. A. (1999) · Nature Cell Biology · doi:10.1038/10054 · Cited by 475
Duensing, S., Lee, L. Y., Duensing, A., Basile, J., Piboonniyom, S.-O., Gonzalez, S., Crum, C. P., & Münger, K. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.170093297 · Cited by 397
RB, the conductor that orchestrates life, death and differentiation
Khidr, L., & Chen, P.-L. (2006) · Oncogene · doi:10.1038/sj.onc.1209612 · Cited by 123
Yissachar, N., Salem, H., Tennenbaum, T., & Motro, B. (2006) · FEBS Letters · doi:10.1016/j.febslet.2006.10.069 · Cited by 118
Centrosome-associated NDR kinase regulates centrosome duplication.
Hergovich, A., Lamla, S., Nigg, E. A., & Hemmings, B. A. (2007) · Molecular Cell · doi:10.1016/j.molcel.2007.01.020 · Cited by 108
Metadata
- Authors
- Lacey, K. R., Jackson, P. K., & Stearns, T.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1999-03-16
- Volume
- 96
- Issue
- 6
- Pages
- 2817-2822
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2115206894
- Cited by
- 427
- References
- 1
- FWCI
- 13.13
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Cyclin-dependent kinase control of centrosome duplication. https://app.cochat.ai/papers/10.1073/pnas.96.6.2817
- The paper
- Lacey, K. R., Jackson, P. K., & Stearns, T. (1999). Cyclin-dependent kinase control of centrosome duplication. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.96.6.2817
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
