The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Biochemical characterization of Wnt-Frizzled interactions using a soluble, biologically active vertebrate Wnt protein
Open accessAbstract
Biochemical studies of Wnt signaling have been hampered by difficulties in obtaining large quantities of soluble, biologically active Wnt proteins. In this paper, we report the production in Drosophila S2 cells of biologically active Xenopus Wnt8 (XWnt8). Epitope- or alkaline phosphatase-tagged XWnt8 proteins are secreted by concentrated S2 cells in a form that is suitable for quantitative biochemical experiments with yields of 5 and 0.5 mg per liter, respectively. Conditions also are described for the production in 293 cells of an IgG fusion of the cysteine-rich domain (CRD) of mouse Frizzled 8 with a yield of 20 mg/liter. We demonstrate the use of these proteins for studying the interactions between soluble XWnt8 and various Frizzled proteins, membrane anchored or secreted CRDs, and a set of insertion mutants in the CRD of Drosophila Frizzled 2. In a solid phase binding assay, the affinity of the XWnt8-alkaline phosphatase fusion for the purified mouse Frizzled 8-CRD-IgG fusion is ≈9 nM.
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Cites
A new secreted protein that binds to Wnt proteins and inhibits their activites
Hsieh, J.-C., Kodjabachian, L., Rebbert, M. L., Rattner, A., Smallwood, P. M., Samos, C. H., Nusse, R., Dawid, I. B., & Nathans, J. (1999) · Nature · doi:10.1038/18899 · Cited by 750
Self-citationFrzb, a Secreted Protein Expressed in the Spemann Organizer, Binds and Inhibits Wnt-8
Wang, S., Krinks, M., Lin, K., Luyten, F. P., & Moos, M. (1997) · Cell · doi:10.1016/s0092-8674(00)81922-4 · Cited by 518
Cited by
The Wnt Signaling Pathway in Development and Disease
Logan, C. Y., & Nusse, R. (2004) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.20.010403.113126 · Cited by 5,325
Wnt/β-Catenin Signaling, Disease, and Emerging Therapeutic Modalities
Nusse, R., & Clevers, H. (2017) · Cell · doi:10.1016/j.cell.2017.05.016 · Cited by 4,342
Wnt proteins are lipid-modified and can act as stem cell growth factors
Willert, K., Brown, J. D., Danenberg, E., Duncan, A. W., Weissman, I. L., Reya, T., Yates, J. R., & Nusse, R. (2003) · Nature · doi:10.1038/nature01611 · Cited by 2,257
A role for Wnt signalling in self-renewal of haematopoietic stem cells
Reya, T., Duncan, A. W., Ailles, L., Domen, J., Scherer, D. C., Willert, K., Hintz, L., Nusse, R., & Weissman, I. L. (2003) · Nature · doi:10.1038/nature01593 · Cited by 2,159
Purified Wnt5a Protein Activates or Inhibits β-Catenin–TCF Signaling Depending on Receptor Context
Mikels, A. J., & Nusse, R. (2006) · PLoS Biology · doi:10.1371/journal.pbio.0040115 · Cited by 1,252
Metadata
- Authors
- Hsieh, J.-C., Rattner, A., Smallwood, P. M., & Nathans, J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1999-03-30
- Volume
- 96
- Issue
- 7
- Pages
- 3546-3551
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2034133599
- Cited by
- 349
- References
- 2
- FWCI
- 4.68
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Hsieh, J.-C., Rattner, A., Smallwood, P. M., & Nathans, J. (1999). Biochemical characterization of Wnt-Frizzled interactions using a soluble, biologically active vertebrate Wnt protein. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.96.7.3546
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