The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
High-efficiency gene transfer into skeletal muscle mediated by electric pulses
Open accessAbstract
Gene delivery to skeletal muscle is a promising strategy for the treatment of muscle disorders and for the systemic secretion of therapeutic proteins. However, present DNA delivery technologies have to be improved with regard to both the level of expression and interindividual variability. We report very efficient plasmid DNA transfer in muscle fibers by using square-wave electric pulses of low field strength (less than 300 V/cm) and of long duration (more than 1 ms). Contrary to the electropermeabilization-induced uptake of small molecules into muscle fibers, plasmid DNA has to be present in the tissue during the electric pulses, suggesting a direct effect of the electric field on DNA during electrotransfer. This i.m. electrotransfer method increases reporter and therapeutic gene expression by several orders of magnitude in various muscles in mouse, rat, rabbit, and monkey. Moreover, i.m. electrotransfer strongly decreases variability. Stability of expression was observed for at least 9 months. With a pCMV-FGF1 plasmid coding for fibroblast growth factor 1, this protein was immunodetected in the majority of muscle fibers subjected to the electric pulses. DNA electrotransfer in muscle may have broad applications in gene therapy and in physiological, pharmacological, and developmental studies.
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Cited by
Nonviral vectors for gene delivery
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Immune evasion in cancer: mechanistic basis and therapeutic strategies
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A PGC-1α isoform induced by resistance training regulates skeletal muscle hypertrophy
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Metadata
- Authors
- Mir, L. M., Bureau, M. F., Gehl, J., Rangara, R., Rouy, D., Caillaud, J.-M., Delaere, P., Branellec, D., Schwartz, B., & Scherman, D.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1999-04-13
- Volume
- 96
- Issue
- 8
- Pages
- 4262-4267
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2018744718
- Cited by
- 914
- References
- 43
- FWCI
- 52.42
- Type
- journal article
- Language
- en
- Open access
- green
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- Mir, L. M., Bureau, M. F., Gehl, J., Rangara, R., Rouy, D., Caillaud, J.-M., Delaere, P., Branellec, D., Schwartz, B., & Scherman, D. (1999). High-efficiency gene transfer into skeletal muscle mediated by electric pulses. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.96.8.4262
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