The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Improved reporter strain for monitoring Cre recombinase-mediated DNA excisions in mice
Open accessAbstract
Effective use of conditional Cre recombinase- loxP gene modification requires Cre-expressing mouse strains with defined patterns of expression. To assess the in vivo functionality of Cre-expressing mice, we have engineered an improved reporter strain for monitoring Cre-mediated excisions. The β-galactosidase-neomycin phosphotransferase fusion gene (βgeo)-trapped ROSA26 locus was modified by gene targeting such that βgeo is expressed only after Cre-mediated excision of loxP -flanked DNA sequences. βgeo from the excised ROSA26 allele is expressed ubiquitously in embryos and adult mice. By mating the reporter strain with Cre-expressing transgenic mice, we have shown that the loxP -flanked ROSA26 allele is accessible to Cre during early embryogenesis, as well as in a specific hematopoietic lineage (T lymphocytes). This improved reporter strain should facilitate monitoring in vivo Cre-mediated excision events in a variety of experimental contexts.
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Schwenk, F., Baron, U., & Rajewsky, K. (1995) · Nucleic Acids Research · doi:10.1093/nar/23.24.5080 · Cited by 1,289
Efficient in vivo manipulation of mouse genomic sequences at the zygote stage.
Lakso, M., Pichel, J. G., Gorman, J. R., Sauer, B., Okamoto, Y., Lee, E., Alt, F. W., & Westphal, H. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.12.5860 · Cited by 1,125
Cited by
Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus
Srinivas, S., Watanabe, T., Lin, C.-S., William, C. M., Tanabe, Y., Jessell, T. M., & Costantini, F. (2001) · BMC Developmental Biology · doi:10.1186/1471-213x-1-4 · Cited by 3,121
Epicardial progenitors contribute to the cardiomyocyte lineage in the developing heart
Zhou, B., Ma, Q., Rajagopal, S., Wu, S. M., Domian, I., Rivera-Feliciano, J., Jiang, D., von Gise, A., Ikeda, S., Chien, K. R., et al. (2008) · Nature · doi:10.1038/nature07060 · Cited by 1,019
Discovery of Endothelial to Mesenchymal Transition as a Source for Carcinoma-Associated Fibroblasts
Zeisberg, E. M., Potenta, S., Xie, L., Zeisberg, M., & Kalluri, R. (2007) · Cancer Research · doi:10.1158/0008-5472.can-07-3127 · Cited by 1,011
Hair follicle stem cells are specified and function in early skin morphogenesis
Nowak, J. A., Polak, L., Pasolli, H. A., & Fuchs, E. (2008) · Cell Stem Cell · doi:10.1016/j.stem.2008.05.009 · Cited by 554
Meikle, L., Talos, D. M., Onda, H., Pollizzi, K., Rotenberg, A., Sahin, M., Jensen, F. E., & Kwiatkowski, D. J. (2007) · The Journal of Neuroscience · doi:10.1523/jneurosci.5540-06.2007 · Cited by 462
Metadata
- Authors
- Mao, X., Fujiwara, Y., & Orkin, S. H.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1999-04-27
- Volume
- 96
- Issue
- 9
- Pages
- 5037-5042
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2048938442
- Cited by
- 329
- References
- 2
- FWCI
- 4.57
- Type
- journal article
- Language
- en
- Open access
- green
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