Hair follicle stem cells are specified and function in early skin morphogenesis
Open accessAbstract
In adult skin, epithelial hair follicle stem cells (SCs) reside in a quiescent niche and are essential for cyclic bouts of hair growth. Niche architecture becomes pronounced postnatally at the start of the first hair cycle. Whether SCs exist or function earlier is unknown. Here we show that slow-cycling cells appear early in skin development, express SC markers, and later give rise to the adult SC population. To test whether these early slow-cycling cells function as SCs, we use Sox9-Cre for genetic marking and K14-Cre to embryonically ablate Sox9, an essential adult SC gene. We find that the progeny of Sox9-expressing cells contribute to all skin epithelial lineages and Sox9 is required for SC specification. In the absence of early SCs, hair follicle and sebaceous gland morphogenesis is blocked, and epidermal wound repair is compromised. These findings establish the existence of early hair follicle SCs and reveal their physiological importance in tissue morphogenesis.
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KRT15 and SOX9 were expressed broadly in the organoid HF outer root sheath as well as the bulge-like region.
“We found that the HF bulge and epithelial stem cells markers, KRT15 and SOX9, were expressed broadly in the organoid HF outer root sheath as well as the bulge-like region (Morris et al., 2004; Nowak et al., 2008; Figure 6B–B″″).”
From Hair Follicle Development in Mouse Pluripotent Stem Cell-Derived Skin Organoids (2018) · cites this paper as Nowak et al., 2008
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- Authors
- Nowak, J. A., Polak, L., Pasolli, H. A., & Fuchs, E.
- Affiliations
- Howard Hughes Medical Institute (US)
- Corresponding author
- Fuchs, Elaine
- Venue
- Cell Stem Cell
- Publisher
- Elsevier BV
- Published
- 2008-07
- Volume
- 3
- Issue
- 1
- Pages
- 33-43
- ISSN
- 1875-9777 (ISSN-L)
- 1934-5909
- PMC
- PMC2877596
- OpenAlex
- W1998479150
- Cited by
- 554
- References
- 3
- Citations by year
- 2026: 18 · 2025: 17 · 2024: 18 · 2023: 25 · 2022: 27 · 2021: 29 · 2020: 30 · 2019: 35 · 2018: 31 · 2017: 25 · 2016: 25 · 2015: 38 · 2014: 45 · 2013: 41 · 2012: 52
- FWCI
- 39.40
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 1878 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Cell stem cell (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Howard Hughes Medical Institute
- National Institutes of Health (2r01ar050452-21)
- Langley Research Center
- National Cancer Institute (T32CA009673)
- National Institute of General Medical Sciences (T32GM007739)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR031737, R01-AR050452)
- Topics
- Health Sciences › Medicine › Urology › Hair Growth and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › dental development and anomalies
- Keywords
- hair follicle stem cells, skin morphogenesis, SOX9, slow-cycling cells, stem cell specification, hair follicle morphogenesis, stem cell niche
- MeSH terms
- Adult, Animals, Cell Cycle, Epithelial Cells, High Mobility Group Proteins, Humans, Morphogenesis, Skin, Skin Physiological Phenomena, Stem Cells, Transcription Factors, Wound Healing, Biomarkers, Hair Follicle, Integrases, Stem Cell Transplantation, Mice, SOX9 Transcription Factor
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