The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
The Nef protein of HIV-1 associates with rafts and primes T cells for activation
Open accessAbstract
The Nef protein is an important virulence factor of primate lentiviruses, yet the mechanisms by which it exerts this influence are imperfectly understood. Here, using an inducible system, we demonstrate that Nef increases IL-2 secretion from T cells stimulated via CD3 or CD28. This effect requires the conservation of the Nef myristoylation signal and SH3-binding proline-based motif. Together with several proteins involved in the initiation and propagation of T cell signaling, Nef associates with membrane microdomains known as rafts. The Nef-mediated superinduction of IL-2 reflects the activation of both NFAT and NFκB. Accordingly, Nef also enhances HIV-1 transcription in response to CD3 or CD28 stimulation. Nef-induced IL-2 hyperresponsiveness is also observed in primary CD4 lymphocytes. Overall, these data suggest that Nef acts at the level of rafts to prime T cells for activation. Likely consequences of this effect are the promotion of HIV-1 replication and the facilitation of virus spread.
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Functional rafts in cell membranes
Simons, K., & Ikonen, E. (1997) · Nature · doi:10.1038/42408 · Cited by 9,757
Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo
Zufferey, R., Nagy, D., Mandel, R. J., Naldini, L., & Trono, D. (1997) · Nature Biotechnology · doi:10.1038/nbt0997-871 · Cited by 1,977
Self-citationCytokine Signals Are Sufficient for HIV-1 Infection of Resting Human T Lymphocytes
Unutmaz, D., KewalRamani, V. N., Marmon, S., & Littman, D. R. (1999) · The Journal of Experimental Medicine · doi:10.1084/jem.189.11.1735 · Cited by 427
Cited by
Cholesterol, lipid rafts, and disease
Simons, K., & Ehehalt, R. (2002) · Journal of Clinical Investigation · doi:10.1172/jci0216390 · Cited by 916
Virus Entry, Assembly, Budding, and Membrane Rafts
Chazal, N., & Gerlier, D. (2003) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.67.2.226-237.2003 · Cited by 482
Role for Human Immunodeficiency Virus Type 1 Membrane Cholesterol in Viral Internalization
Guyader, M., Kiyokawa, E., Abrami, L., Turelli, P., & Trono, D. (2002) · Journal of Virology · doi:10.1128/jvi.76.20.10356-10364.2002 · Cited by 170
Self-citationRole of viral regulatory and accessory proteins in HIV-1 replication
Seelamgari, A. (2004) · Frontiers in Bioscience · doi:10.2741/1403 · Cited by 98
Metadata
- Authors
- Wang, J.-K., Kiyokawa, E., Verdin, E., & Trono, D.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2000-01-04
- Volume
- 97
- Issue
- 1
- Pages
- 394-399
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1981298192
- Cited by
- 276
- References
- 3
- FWCI
- 10.59
- Type
- journal article
- Language
- en
- Open access
- green
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- Wang, J.-K., Kiyokawa, E., Verdin, E., & Trono, D. (2000). The Nef protein of HIV-1 associates with rafts and primes T cells for activation. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.97.1.394
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