The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
A mitochondrial ferredoxin is essential for biogenesis of cellular iron-sulfur proteins
Open accessAbstract
Iron-sulfur (Fe/S) cluster-containing proteins catalyze a number of electron transfer and metabolic reactions. The components and molecular mechanisms involved in the assembly of the Fe/S clusters have been identified only partially. In eukaryotes, mitochondria have been proposed to execute a crucial task in the generation of intramitochondrial and extramitochondrial Fe/S proteins. Herein, we identify the essential ferredoxin Yah1p of Saccharomyces cerevisiae mitochondria as a central component of the Fe/S protein biosynthesis machinery. Depletion of Yah1p by regulated gene expression resulted in a 30-fold accumulation of iron within mitochondria, similar to what has been reported for other components involved in Fe/S protein biogenesis. Yah1p was shown to be required for the assembly of Fe/S proteins both inside mitochondria and in the cytosol. Apparently, at least one of the steps of Fe/S cluster biogenesis within mitochondria requires reduction by ferredoxin. Our findings lend support to the idea of a primary function of mitochondria in the biosynthesis of Fe/S proteins outside the organelle. To our knowledge, Yah1p is the first member of the ferredoxin family for which a function in Fe/S cluster formation has been established. A similar role may be predicted for the bacterial homologs that are encoded within iron-sulfur cluster assembly ( isc ) operons of prokaryotes.
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Cited by
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Mitochondrial remnant organelles of Giardia function in iron-sulphur protein maturation
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Grx5 Is a Mitochondrial Glutaredoxin Required for the Activity of Iron/Sulfur Enzymes
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Mechanisms of Mitochondrial Iron-Sulfur Protein Biogenesis
Lill, R., & Freibert, S.-A. (2020) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-013118-111540 · Cited by 453
Self-citation
Metadata
- Authors
- Lange, H., Kaut, A., Kispal, G., & Lill, R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2000-02
- Volume
- 97
- Issue
- 3
- Pages
- 1050-1055
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1978004953
- Cited by
- 272
- References
- 43
- FWCI
- 27.32
- Type
- journal article
- Language
- en
- Open access
- green
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