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Cited by 323

The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.

Abnormal spermatogenesis and reduced fertility in transition nuclear protein 1-deficient mice

Open access
Yu, Y. Eugene, Zhang, Yun, Unni, Emmanual, Shirley, Cynthia R., Deng, Jian M., Russell, Lonnie D., Weil, Michael M., Behringer, Richard R., Meistrich, Marvin L.·2000·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.97.9.4683
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Abstract

Transition nuclear proteins (TPs), the major proteins found in chromatin of condensing spermatids, are believed to be important for histone displacement and chromatin condensation during mammalian spermatogenesis. We generated mice lacking the major TP, TP1, by targeted deletion of the Tnp1 gene in mouse embryonic stem cells. Surprisingly, testis weights and sperm production were normal in the mutant mice, and only subtle abnormalities were observed in sperm morphology. Electron microscopy revealed large rod-like structures in the chromatin of mutant step 13 spermatids, in contrast to the fine chromatin fibrils observed in wild type. Steps 12–13 spermatid nuclei from the testis of Tnp1-null mice contained, in place of TP1, elevated levels of TP2 and some protamine 2 (P2) precursor. Most of the precursor was processed to mature P2, but high levels of incompletely processed forms remained in epididymal spermatozoa. Sperm motility was reduced severely, and ≈60% of Tnp1-null males were infertile. We concluded that TP1 is not essential for histone displacement or chromatin condensation. The absence of TP1 may partially be compensated for by TP2 and P2 precursor, but this dysregulation of nucleoprotein replacement results in an abnormal pattern of chromatin condensation and in reduced fertility.

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Metadata

Authors
Yu, Y. E., Zhang, Y., Unni, E., Shirley, C. R., Deng, J. M., Russell, L. D., Weil, M. M., Behringer, R. R., & Meistrich, M. L.
Venue
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Published
2000-04-25
Volume
97
Issue
9
Pages
4683-4688
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.97.9.4683
OpenAlex
W2151499107
Cited by
323
References
16
FWCI
14.57
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Abnormal spermatogenesis and reduced fertility in transition nuclear protein 1-deficient mice. https://app.cochat.ai/papers/10.1073/pnas.97.9.4683
The paper
Yu, Y. E., Zhang, Y., Unni, E., Shirley, C. R., Deng, J. M., Russell, L. D., Weil, M. M., Behringer, R. R., & Meistrich, M. L. (2000). Abnormal spermatogenesis and reduced fertility in transition nuclear protein 1-deficient mice. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.97.9.4683

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