Epiregulin
Open accessAbstract
Epiregulin, a novel epidermal growth factor (EGF)-related growth regulating peptide, was purified from conditioned medium of the mouse fibroblast-derived tumor cell line NIH3T3/clone T7. It was a 46-amino-acid single chain polypeptide, and its amino acid sequence exhibited 24-50% amino acid sequence identity with sequences of other EGF-related growth factors. Epiregulin exhibited bifunctional regulatory properties: it inhibited the growth of several epithelial tumor cells and stimulated the growth of fibroblasts and various other types of cells. Epiregulin bound to the EGF receptors of epidermoid carcinoma A431 cells much more weakly than did EGF, but was nevertheless much more potent than EGF as a mitogen for rat primary hepatocytes and Balb/c 3T3 A31 fibroblasts. These findings suggest that epiregulin plays important roles in regulating the growth of epithelial cells and fibroblasts by binding to receptors for EGF-related ligands.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Epiregulin is an epidermal growth factor family member with a potent mitogenic effect on hepatocytes.
“Epiregulin is an epidermal growth factor family member with a potent mitogenic effect on hepatocytes82.”
From The gut microbiome and liver cancer: mechanisms and clinical translation (2017) · cites this paper as [82]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Molecular cloning and expression of human hepatocyte growth factor
Nakamura, T., Nishizawa, T., Hagiya, M., Seki, T., Shimonishi, M., Sugimura, A., Tashiro, K., & Shimizu, S. (1989) · Nature · doi:10.1038/342440a0 · Cited by 2,158 · see citing works
Aaronson, S. A. (1991) · Science · doi:10.1126/science.1659742 · Cited by 2,017 · see citing works
Coussens, L. M., Yang‐Feng, T. L., Liao, Y.-C., Chen, E., Gray, A., McGrath, J. P., Seeburg, P. H., Libermann, T. A., Schlessinger, J., Francke, U., et al. (1985) · Science · doi:10.1126/science.2999974 · Cited by 1,815 · see citing works
Autocrine growth factors and cancer
Sporn, M. B., & Roberts, A. B. (1985) · Nature · doi:10.1038/313745a0 · Cited by 1,458 · see citing works
Autocrine Secretion and Malignant Transformation of Cells
Sporn, M. B., & Todaro, G. J. (1980) · New England Journal of Medicine · doi:10.1056/nejm198010093031511 · Cited by 1,363 · see citing works
Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor
Yamamoto, T. S., Ikawa, S., Akiyama, T., Semba, K., Nomura, N., Miyajima, N., Saito, T., & Toyoshima, K. (1986) · Nature · doi:10.1038/319230a0 · Cited by 1,204 · see citing works
A heparin-binding growth factor secreted by macrophage-like cells that is related to EGF
Higashiyama, S., Abraham, J. A., Miller, J., Fiddes, J. C., & Klagsbrun, M. (1991) · Science · doi:10.1126/science.1840698 · Cited by 1,127 · see citing works
The neu oncogene encodes an epidermal growth factor receptor-related protein
Bargmann, C. I., Hung, M., & Weinberg, R. A. (1986) · Nature · doi:10.1038/319226a0 · Cited by 1,115 · see citing works
Identification of Heregulin, a Specific Activator of p185 erbB2
Holmes, W. E., Sliwkowski, M. X., Akita, R. W., et al. (1992) · Science · doi:10.1126/science.256.5060.1205 · Cited by 965 · see citing works
Growth Inhibitor from BSC-1 Cells Closely Related to Platelet Type β Transforming Growth Factor
Tucker, R. F., Shipley, G. D., Moses, H. L., & Holley, R. W. (1984) · Science · doi:10.1126/science.6093254 · Cited by 768 · see citing works
Sandgren, E. P., Luetteke, N., Palmiter, R. D., Brinster, R. L., & Lee, D. C. (1990) · Cell · doi:10.1016/0092-8674(90)90075-p · Cited by 752 · see citing works
Kraus, M. H., Issing, W. J., Takashi, M. I. K. I., Popescu, N. C., & Aaronson, S. A. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.23.9193 · Cited by 737 · see citing works
Plowman, G. D., Culouscou, J., Whitney, G. S., Green, J. M., Carlton, G. W., Foy, L., NEUBAUER, M., & SHOYAB, M. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.5.1746 · Cited by 714 · see citing works
Jhappan, C., Stahle, C., Harkins, R. N., Fausto, N., Smith, G. H., & Merlino, G. T. (1990) · Cell · doi:10.1016/0092-8674(90)90076-q · Cited by 709 · see citing works
Rat Transforming Growth Factor Type 1: Structure and Relation to Epidermal Growth Factor
Marquardt, H., Hunkapiller, M. W., Hood, L., & Todaro, G. J. (1984) · Science · doi:10.1126/science.6320373 · Cited by 657 · see citing works
Structure and Function of Human Amphiregulin: A Member of the Epidermal Growth Factor Family
SHOYAB, M., Plowman, G. D., McDonald, V. L., Bradley, J. G., & Todaro, G. J. (1989) · Science · doi:10.1126/science.2466334 · Cited by 597 · see citing works
Wen, D., Peles, E., Cupples, R. L., et al. (1992) · Cell · doi:10.1016/0092-8674(92)90456-m · Cited by 578 · see citing works
The Primary Structure of Epidermal Growth Factor
Savage, C. R., Inagami, T., & Cohen, S. (1972) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)44569-9 · Cited by 539 · see citing works
Mead, J. E., & Fausto, N. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.5.1558 · Cited by 535 · see citing works
Development of mammary hyperplasia and neoplasia in MMTV-TGFα transgenic mice
Matsui, Y., Halter, S. A., Holt, J. T., Hogan, B. L. M., & Coffey, R. J. (1990) · Cell · doi:10.1016/0092-8674(90)90077-r · Cited by 440 · see citing works
Oncostatin M: a growth regulator produced by differentiated histiocytic lymphoma cells.
Zarling, J. M., SHOYAB, M., Marquardt, H., Hanson, M., Lioubin, M. N., & Todaro, G. J. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.24.9739 · Cited by 435 · see citing works
Betacellulin: a mitogen from pancreatic beta cell tumors
Shing, Y., Christofori, G., Hanahan, D., Ono, Y., Sasada, R., Igarashi, K., & Folkman, J. (1993) · Science · doi:10.1126/science.8456283 · Cited by 389 · see citing works
Molecular cloning and expression of an additional epidermal growth factor receptor-related gene.
Plowman, G. D., Whitney, G. S., NEUBAUER, M., Green, J. M., McDonald, V. L., Todaro, G. J., & SHOYAB, M. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.13.4905 · Cited by 366 · see citing works
SHOYAB, M., McDonald, V. L., Bradley, J. G., & Todaro, G. J. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.17.6528 · Cited by 363 · see citing works
Biochemical Studies on Liver Functions in Primary Cultured Hepatocytes of Adult Rats1
Tanaka, K., Mitsuru, S. A. T. O., Tomita, Y., & Ichihara, A. (1978) · The Journal of Biochemistry · doi:10.1093/oxfordjournals.jbchem.a132207 · Cited by 321 · see citing works
Differential expression of epidermal growth factor-related proteins in human colorectal tumors.
Ciardiello, F., Kim, N., Saeki, T., Dono, R., Persico, M. G., Plowman, G. D., Garrigues, J., Radke, S., Todaro, G. J., & Salomon, D. S. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.17.7792 · Cited by 267 · see citing works
BROWN, J. P., Twardzik, D. R., Marquardt, H., et al. (1985) · Nature · doi:10.1038/313491a0 · Cited by 250 · see citing works
Purification and characterization of vaccinia virus growth factor
Stroobant, P., Rice, A. P., Gullick, W. J., et al. (1985) · Cell · doi:10.1016/s0092-8674(85)80133-1 · Cited by 235 · see citing works
Yanagita, K., Nagaike, M., Ishibashi, H., Niho, Y., Matsumoto, K., & Nakamura, T. (1992) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(92)91803-x · Cited by 214 · see citing works
Identity of a tumor cytotoxic factor from human fibroblasts and hepatocyte growth factor
Higashio, K., Shima, N., Goto, M., et al. (1990) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(90)91287-3 · Cited by 194 · see citing works
Lü, C., Vickers, M. F., & Kerbel, R. S. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.19.9215 · Cited by 170 · see citing works
Abraham, J. A., Damm, D. L., Bajardi, A., et al. (1993) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1993.1020 · Cited by 159 · see citing works
Johnson, G. R., Saeki, T., Gordon, A. W., SHOYAB, M., Salomon, D. S., & Stromberg, K. (1992) · The Journal of Cell Biology · doi:10.1083/jcb.118.3.741 · Cited by 150 · see citing works
Kinoshita, T., Hirao, S., Matsumoto, K., et al. (1991) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(91)91987-n · Cited by 145 · see citing works
Heparin-Binding EGF-like Growth-Factor Is a Potent Mitogen for Rat Hepatocytes
Ito, N., Kawata, S., Tamura, S., Kiso, S., Tsushima, H., Damm, D. L., Abraham, J. A., Higashiyama, S., Taniguchi, N., & Matsuzawa, Y. (1994) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1994.1004 · Cited by 92 · see citing works
Ziober, B. L., Willson, J. K. V., Hymphrey, L. E., et al. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)54207-1 · Cited by 84 · see citing works
Larco, J. E. D., Reynolds, R. K., Carlberg, K., et al. (1980) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)85758-7 · Cited by 82 · see citing works
Oncostatin M is a mitogen for rabbit vascular smooth muscle cells.
Grove, R. I., Eberhardt, C., Abid, S., Mazzucco, C. E., Liu, J., Kiener, P. A., Todaro, G. J., & SHOYAB, M. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.3.823 · Cited by 82 · see citing works
Engler, D. A., Matsunami, R. K., Campion, S. R., et al. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)37767-6 · Cited by 71 · see citing works
Ray, P., Moy, F. J., Montelione, G. T., et al. (1988) · Biochemistry · doi:10.1021/bi00419a017 · Cited by 47 · see citing works
Hirose, M., Takahashi, N., Oe, H., et al. (1988) · Analytical Biochemistry · doi:10.1016/0003-2697(88)90028-0 · Cited by 26 · see citing works
Lazar, E., Watanabe, S., Dalton, S., et al. (1988) · Molecular and Cellular Biology · doi:10.1128/mcb.8.3.1247 · Cited by 22 · see citing works
Flagged first, then by citations
Research works printed but not linked: 9
- Zarling, J. M., Shoyab, M., Marquardt, H., Harson, M. B., Lioubin, M. N., and Todaro, G. J. (1986) Proc. Natl. Acad. Sci. U. S. A. 83,9739-9743
- Shyoab, M., McDonald, V. D., Bradley, J. G., and Todaro, G. J. (1988) Proc. Natl. Acad. Sci. U. S. A. 85, 6528-6532
- Lu, C., Vickers, M. F., and Krebel, R. S. (1991) Proc. Natl. Acad. Sci. U. S. A. 89, 9215-9219
- Grobe, R. I., Eberhardt, C., Abid, S., Mazzucco., Liv, J., Kiener, P., Todaro, G. J., and Shyoab, M. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 823-827
- Kraus, M. H., Issing, W., Miki, T., Popescu, N. C., and Aronson, S. A. (1989) Proc. Natl. Acad. Sci. U. S. A. 86, 9193-9197
- Plowman, G. J., Whitney, G. S., Neubauer, M. G., Green, J. M., McDonald, V. L., Todaro, G. J., and Shyoab, M. (1990) Proc. Nat/. Acad. Sci. U. S. A. 87, 4905-4909
- Plowman, G. J., Culouscou, J.-M., Whitney, G. S., Green, J. M., Carlton, G. W., Neubauer, M. G., and Shoyab, M. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 1746-1750
- Mead, J. E., and Fausto, N. (1989) Proc. Natl. Acad. Sci. U. S. A. 86, 1558- 1562
- Ciardiello, F., Kim, N., Saeki, T., Dono, R, Persico, M. G., Plowman, G. D., Garrigues, J., Radke, S., Todaro, G. J., and Salomon, D. S. (1991) Proc. Nat/. Acad. Sci. U. S. A. 7792-7796
Cited by
Sorted by citations
Promotion of Hepatocellular Carcinoma by the Intestinal Microbiota and TLR4
Dapito, D. H., Mencin, A., Gwak, G.-Y., Pradere, J.-P., Jang, M.-K., Mederacke, I., Caviglia, J. M., Khiabanian, H., Adeyemi, A., Bataller, R., et al. (2012) · Cancer Cell · doi:10.1016/j.ccr.2012.02.007 · Cited by 1,346 · see citing works
The Role of Cancer-Associated Fibroblasts and Fibrosis in Liver Cancer
Affo, S., Yu, L.-X., & Schwabe, R. F. (2017) · Annual Review of Pathology: Mechanisms of Disease · doi:10.1146/annurev-pathol-052016-100322 · Cited by 757 · see citing works
The gut microbiome and liver cancer: mechanisms and clinical translation
Yu, L.-X., & Schwabe, R. F. (2017) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/nrgastro.2017.72 · Cited by 634 · see citing works
Reyes, N. S., Krasilnikov, M., Allen, N. C., Lee, J. Y., Hyams, B., Zhou, M., Ravishankar, S., Cassandras, M., Wang, C., Khan, I., et al. (2022) · Science · doi:10.1126/science.abf3326 · Cited by 181 · see citing works
Sen, M., Wang, X., Hamdan, F. H., Rapp, J., Eggert, J., Kosinsky, R. L., Wegwitz, F., Kutschat, A. P., Younesi, F. S., Gaedcke, J., et al. (2019) · Clinical Epigenetics · doi:10.1186/s13148-019-0690-5 · Cited by 61 · see citing works
- Authors
- Isobe, T., Okuyama, T., Toyoda, H., Komurasaki, T., Uchida, D., Takayama, Y., & Hanada, K.
- Affiliations
- Taisho Pharmaceutical (Japan) (JP)
- Tokyo Metropolitan University (JP)
- Corresponding author
- Komurasaki, Toshi
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1995-03
- Volume
- 270
- Issue
- 13
- Pages
- 7495-7500
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1607351680
- Cited by
- 269
- References
- 42
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 6 · 2023: 4 · 2022: 6 · 2021: 6 · 2020: 7 · 2019: 8 · 2018: 4 · 2017: 7 · 2016: 4 · 2015: 2 · 2014: 14 · 2013: 9 · 2012: 10
- FWCI
- 6.43
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Oncology › HER2/EGFR in Cancer Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fibroblast Growth Factor Research
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Keywords
- epiregulin, epidermal growth factor receptor, NIH3T3 cells, tumor cell growth inhibition, fibroblast proliferation, receptor binding, amino acid sequence, cell growth regulation
- MeSH terms
- Amino Acid Sequence, Animals, Carcinoma, Squamous Cell, Cell Division, Cell Line, Cells, Cultured, Chromatography, Chromatography, Gel, Chromatography, High Pressure Liquid, Chromatography, Ion Exchange, Epidermal Growth Factor, HeLa Cells, Humans, Liver, Mice, Inbred BALB C, Molecular Sequence Data, Tumor Cells, Cultured, 3T3 Cells, Sequence Homology, Amino Acid, Durapatite, Mice, Rats, ErbB Receptors, Epiregulin
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
