A heparin-binding growth factor secreted by macrophage-like cells that is related to EGF
Abstract
Macrophage-like U-937 cells secrete a 22-kilodalton heparin-binding growth factor that is mitogenic for BALB-3T3 fibroblasts and smooth muscle cells, but not endothelial cells. The amino acid sequence predicted from complementary DNA clones indicates that the mitogen is a new member of the epidermal growth factor (EGF) family. This heparin-binding EGF-like growth factor (HB-EGF) binds to EGF receptors on A-431 epidermoid carcinoma cells and smooth muscle cells, but is a far more potent mitogen for smooth muscle cells than is EGF. HB-EGF is also expressed in cultured human macrophages and may be involved in macrophage-mediated cellular proliferation.
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pro-HB-EGF is made as a transmembrane protein and metalloproteinases can cleave it in a juxtamembrane domain to release soluble HB-EGF.
“It is first synthesized as a transmembrane protein (pro-HB-EGF) and can be cleaved by metalloproteinases (MMPs) within a juxtamembrane domain on the cell surface to release soluble HB-EGF (Higashiyama et al., 1991; Higashiyama et al., 1992; Higashiyama and Nanba, 2005).”
From HB-EGF is Necessary and Sufficient for Müller Glia Dedifferentiation and Retina Regeneration (2012) · cites this paper as Higashiyama et al., 1991
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“It is first synthesized as a transmembrane protein (pro-HB-EGF) and can be cleaved by metalloproteinases (MMPs) within a juxtamembrane domain on the cell surface to release soluble HB-EGF (Higashiyama et al., 1991; Higashiyama et al., 1992; Higashiyama and Nanba, 2005).”
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Self-citation
- Authors
- Higashiyama, S., Abraham, J. A., Miller, J., Fiddes, J. C., & Klagsbrun, M.
- Affiliations
- Boston Children's Hospital (US)
- California Biotechnology Inc., Mountain View, CA 94043.
- ORCID
- Higashiyama, Shigeki
- Venue
- Science
- Published
- 1991-02-22
- Volume
- 251
- Issue
- 4996
- Pages
- 936-939
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2038086562
- Cited by
- 1,127
- References
- 38
- Citations by year
- 2026: 2 · 2025: 10 · 2024: 13 · 2023: 10 · 2022: 14 · 2021: 5 · 2020: 13 · 2019: 12 · 2018: 16 · 2017: 17 · 2016: 13 · 2015: 21 · 2014: 23 · 2013: 32 · 2012: 37
- FWCI
- 34.28
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute (P01CA045548, CA 37392, CA-45548)
- Topics
- Health Sciences › Medicine › Oncology › HER2/EGFR in Cancer Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fibroblast Growth Factor Research
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Keywords
- HB-EGF, epidermal growth factor family, macrophages, EGF receptor, smooth muscle cells, mitogenic activity, fibroblasts, cell proliferation, A431 cells
- MeSH terms
- Amino Acid Sequence, Cell Division, Cell Line, Chromatography, Affinity, Chromatography, High Pressure Liquid, DNA Replication, Epidermal Growth Factor, Growth Substances, Heparin, Humans, Kinetics, Macrophages, Molecular Sequence Data, Protein Binding, Sequence Homology, Nucleic Acid, ErbB Receptors
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