The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Ca2+ Regulates the Interaction between Synaptotagmin and Syntaxin 1
Open accessAbstract
While there is compelling evidence that the synaptic vesicle protein synaptotagmin serves as the major Ca2+ sensor for regulated exocytosis, it is not known how Ca2+ binding initiates membrane fusion. Here we report that Ca2+ increases the affinity, by approximately 2 orders of magnitude, between synaptotagmin and syntaxin 1, a component of the synaptic fusion apparatus. This effect is specific for divalent cations which can stimulate exocytosis of synaptic vesicles (Ca2+ > Ba2+, Sr2+ >> Mg2+). The Ca(2+)-dependence of the interaction was composed of two components with EC50 values of 0.7 and 180 microM Ca2+. The interaction is mediated by the carboxyl-terminal region of syntaxin 1 (residues 194-288) and is regulated by a novel Ca(2+)-binding site(s) which does not require phospholipids and is not disrupted by mutations that abolish Ca(2+)-dependent phospholipid binding to synaptotagmin. We propose that this interaction constitutes an essential step in excitation-secretion coupling.
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Synaptotagmin I: A major Ca2+ sensor for transmitter release at a central synapse
Geppert, M., Goda, Y., Hammer, R. E., Li, C., Rosahl, T. W., Stevens, C. F., & Südhof, T. C. (1994) · Cell · doi:10.1016/0092-8674(94)90556-8 · Cited by 1,483
A single C2 domain from synaptotagmin I is sufficient for high affinity Ca2+/phospholipid binding.
Davletov, B. A., & Südhof, T. C. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)74326-9 · Cited by 480 · cited 3×
“As mentioned above, this contrasts with the finding that purified synaptotagmin binds Ca21 only in the presence of phospholipids (Brose et al., 1992), a property conferred by the first C2-domain (Davletov and Südhof, 1993; Chapman and Jahn, 1994a; Fukuda et al., 1994).”
BackgroundChapman, E. R., An, S., Barton, N., & Jahn, R. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)47003-2 · Cited by 295
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Cited by
Südhof, T. C. (2004) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.26.041002.131412 · Cited by 2,547
Membrane Fusion: Grappling with SNARE and SM Proteins
Südhof, T. C., & Rothman, J. E. (2009) · Science · doi:10.1126/science.1161748 · Cited by 2,027
Membrane Fusion and Exocytosis
Jahn, R., & Südhof, T. C. (1999) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.68.1.863 · Cited by 1,259
Self-citationSynaptotagmin I functions as a calcium regulator of release probability
Fernández-Chacón, R., Königstorfer, A., Gerber, S. H., García, J., Matos, M. F., Stevens, C. F., Brose, N., Rizo, J., Rosenmund, C., & Südhof, T. C. (2001) · Nature · doi:10.1038/35065004 · Cited by 961
Burgoyne, R. D., & Morgan, A. (2003) · Physiological Reviews · doi:10.1152/physrev.00031.2002 · Cited by 934
Metadata
- Authors
- Chapman, E. R., Hanson, P. I., An, S., & Jahn, R.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1995-10
- Volume
- 270
- Issue
- 40
- Pages
- 23667-23671
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1970930136
- Cited by
- 376
- References
- 3
- FWCI
- 7.21
- Type
- journal article
- Language
- en
- Open access
- hybrid
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